2010 Fiscal Year Final Research Report
Molecular basis for the formation of translational repression complex that regulates stem cell differentiation
| Project/Area Number |
20570111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
NAGATA Takashi Yokohama City University, 生命ナノシステム科学研究科, 助教 (10415250)
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| Co-Investigator(Kenkyū-buntansha) |
KATAHIRA Masato 京都大学, エネルギー理工学研究所, 教授 (70211844)
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| Project Period (FY) |
2008 – 2010
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| Keywords | Musashi / 翻訳抑制 / 幹細胞 / 分化制御 / RNA結合タンパク質 / NMR / RBD / 溶液構造 |
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| Research Abstract |
Musashi1 (Msi1) is a neural protein that suppresses translation of target mRNAs and contributes in maintenance of cell stemness and tumorigenesis. We have determined the RNA recognition mechanism by Msi1. Minimal RNA sequence for Msi1, and the binding mechanism of Msi1 that we have elucidated would provide important clues to discover new target genes for Msi1. Meanwhile, we have determined the structure of PABP and identified its Msi1 binding sites. Finally, we have prepared a protocol for building protein and nucleic acid complex models that would support the structural analysis of translational repression complex.
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