2010 Fiscal Year Final Research Report

Sequence-specificity and binding-cooperativity of multiple DNA-binding domains of CUT-homeodomain transcription factors

Research Project

Project/Area Number	20570119
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Structural biochemistry
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	YAMASAKI Kazuhiko National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 主任研究員 (00358243)
Project Period (FY)	2008 – 2010
Keywords	DNA結合タンパク質 / 協同性と競合性 / 転写制御 / 免疫細胞分化 / NMR分光解析 / 等温滴定熱量分析 / 認識コンセンサス配列
Research Abstract	The DNA-binding mechanism by multiple DNA-binding domains of CUT-homeodomain transcription factors were analyzed by methods of structural biology and physical chemistry. NMR and isothermal titration calorimetry analyses revealed that CUTr1 domain and homeodomain of transcription factor SATB1 bind to DNA containing TAATA sequence in a cooperative manner. A SELEX experiment showed that CUTr1 has the major role in determining the sequence specificity.

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