2010 Fiscal Year Final Research Report
Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor.
| Project/Area Number |
20570120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
AKIYAMA Masashi National Cardiovascular Center Research Institute, 分子病態部, 室長 (30298179)
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| Co-Investigator(Kenkyū-buntansha) |
KOKAME Koichi 独立行政法人国立循環器病研究センター, 分子病態部, 室長 (40270730)
TAKEDA Soichi 独立行政法人国立循環器病研究センター, 分子病態部, 室長 (80332279)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分子認識及び相互作用 |
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| Research Abstract |
We determined crystal structures of an exosite-containing human ADAMTS13 fragment (residues 287-685) required for the cleavage of von Willebrand factor (VWF). We identified 3 VWF-binding exosites on the linearly aligned discontinuous surfaces of the multiple domains traversing the W-shaped molecule. This arrangement suggests that these exosites bind collaboratively to multiple discontinuous regions of VWF.
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