2010 Fiscal Year Final Research Report
Studies on the NFkB activation via diacylglycerol kinase alpha
| Project/Area Number |
20570135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KAI Masahiro Sapporo Medical University, 医学部, 講師 (80260777)
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| Co-Investigator(Kenkyū-buntansha) |
SAKANE Fumio 札幌医科大学, 医学部, 准教授 (10183815)
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| Project Period (FY) |
2008 – 2010
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| Keywords | NFκB / アポトーシス / ジアシルグリセロールキナーゼ / メラノーマ |
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| Research Abstract |
Using human melanoma cells, we demonstrated that diacylglycerol kinase (DGK) alpha enhanced tumor necrosis factor-alpha (TNF-alpha)-induced activation of nuclear factor-kappaB (NF-kappaB) via protein kinase Czeta-dependent phosphorylation of a large subunit of NF-kappaB, p65/RelA. We found that the both of DGKalpha-specfic siRNA and TNF-alpha significantly attenuated the melanoma tumor growth in mice. DGKalpha is suggested to be a novel therapeutic target for melanoma.
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