2010 Fiscal Year Final Research Report
Molecular mechanism of clock regulation and signal transduction by a heme sensor transcription factor, NPAS2
| Project/Area Number |
20570139
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
SAGAMI Ikuko Kyoto Prefectural University, 大学院・生命環境科学研究科, 教授 (10143033)
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| Project Period (FY) |
2008 – 2010
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| Keywords | シグナル伝達 / 分子認識 / 金属タンパク質 / 体内時計 / DNA結合活性 / ガス小分子 |
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| Research Abstract |
We analyzed the effects of gaseous small molecules on function of NPAS2, a heme protein associated with mammalian circadian rhythms. The transcriptional activity and DNA binding activity of NPAS2 were affected in a concentration-dependent-manner by CO or NO, suggesting cross talk between circadian rhythm and CO-, NO-signaling system. The analysis of mutants revealed that specific amino-acid residues in the PASA heme domain are essential to signal transduction in the molecule.
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