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2010 Fiscal Year Final Research Report

Single motor mechanics of bacterial flagellar motor

Research Project

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Project/Area Number 20570147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

INOUE Yuichi  東北大学, 多元物質科学研究所, 助教 (50323499)

Co-Investigator(Renkei-kenkyūsha) ISHIJIMA Akihiko  東北大学, 多元物質科学研究所, 教授 (80301216)
FUKUOKA Hajime  東北大学, 多元物質科学研究所, 助教 (50447190)
Project Period (FY) 2008 – 2010
Keywords生物物理 / 分子モーター / マイクロ・ナノデバイス
Research Abstract

High speed measurement of rotation of the bacterial flagellar motor was made to understand the molecular mechanism of force generation. Using a new system for temperature control, we found the torque steps induced by the heating and the recovery steps by restoring temperature, suggesting dynamics of torque-generating units in a motor.

  • Research Products

    (34 results)

All 2011 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (22 results) Book (4 results) Remarks (1 results)

  • [Journal Article] Ishijima, Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell2011

    • Author(s)
      Shun Terasawa, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Hiroto Takahashi, and Akihiko
    • Journal Title

      Biophysical Journal

      Volume: vol 100 Pages: 2193-2200

    • Peer Reviewed
  • [Journal Article] wo methods of temperature control for single-molecule measurements2011

    • Author(s)
      Matthew A. B. Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M. Berry, T
    • Journal Title

      European Biophysics Journal

      Volume: vol 40 Pages: 651-660

    • Peer Reviewed
  • [Journal Article] Adhesion Effects of a Guanidinium Ion Appended Dendritic"Molecular Glue" on the ATP-Driven Sliding Motion of Actomyosin2010

    • Author(s)
      Kou Okuro, Kazushi Kinbara, Kuniaki Takeda, Yuichi Inoue, Akihiko Ishijima, Takuzo Aida
    • Journal Title

      Angewandte Chemie, International Edition

      Volume: vol 49 Pages: 3030-3033

    • Peer Reviewed
  • [Journal Article] Exchange of rotor components in functioning bacterial flagellar motor2010

    • Author(s)
      Hajime Fukuoka, Yuichi Inoue, Shun Terasawa, Hiroto Takahashi, Akihiko Ishijima
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: vol 394 Pages: 130-135

    • Peer Reviewed
  • [Journal Article] Thermosensing Function of the Escherichia coli Redox Sensor Aer2010

    • Author(s)
      So-ichiro Nishiyama, Shinji Ohno, Noriko Ohta, Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima, Ikuro Kawagishi
    • Journal Title

      Journal of Bacteriology

      Volume: vol 192 Pages: 1740-1743

    • Peer Reviewed
  • [Journal Article] Verification of Single-Molecule Imaging and Single-Molecule Measurements2010

    • Author(s)
      Akihiko Ishijima, Hajime Fukuoka, Yuichi Inoue
    • Journal Title

      Journal of Robotics and Mechatronics

      Volume: vol 22 Pages: 568-578

    • Peer Reviewed
  • [Journal Article] Torque-Speed Relationships of Na+-driven Chimeric Flagellar Motors in Escherichia coli2008

    • Author(s)
      Yuichi Inoue, Chien-Jung Lo, Hajime Fukuoka, Hiroto Takahashi, Yoshiyuki Sowa, Teuta Pilizota, George H. Wadhams, Michio Homma, Richard M. Berry, Akihiko Ishijima
    • Journal Title

      Journal of Molecular Biology

      Volume: vol 376 Pages: 1251-1259

    • Peer Reviewed
  • [Presentation] バクテリアべん毛モーター運動の光制御2011

    • Author(s)
      井上裕一
    • Organizer
      FIRST川合プロジェクト2010年度末報告会
    • Place of Presentation
      東京
    • Year and Date
      2011-02-18
  • [Presentation] Torque steps of the bacterial flagellar motor induced by temperature control2011

    • Author(s)
      Yuichi Inoue
    • Organizer
      International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
    • Place of Presentation
      京都
    • Year and Date
      2011-01-25
  • [Presentation] Torque steps of the bacterial flagellar motor induced by heating2011

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      BLAST XI meeting (Bacterial Locomotion and Signal Transduction
    • Place of Presentation
      USA, New Orleans
    • Year and Date
      2011-01-21
  • [Presentation] 生体分子モーター活性の局所的熱変調2010

    • Author(s)
      井上裕一, 福岡創, 石島秋彦
    • Organizer
      第36回日本生体エネルギー研究会・特定領域研究「革新的ナノバイオ」合同シンポジウム
    • Place of Presentation
      吹田市
    • Year and Date
      2010-11-18
  • [Presentation] Analysis of torque steps of the bacterial flagellar motor induced by heating2010

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-22
  • [Presentation] 生物物理にとっての高速カメラ革新2010

    • Author(s)
      井上裕一
    • Organizer
      第48回日本生物物理学会年会ランチョンセミナー
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-20
  • [Presentation] バクテリアべん毛モーターの温度応答解析と局所的熱変調2010

    • Author(s)
      井上裕一, 福岡創, 石島秋彦
    • Organizer
      特定領域研究「膜超分子モーターの革新的ナノサイエンス」第5回班会議
    • Place of Presentation
      東京
    • Year and Date
      2010-06-24
  • [Presentation] Torque steps in the temperature response of the chimeric bacterial flagellar motor2010

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      International Symposium of Joint Research Network on Advanced Materials and Devices 「彫」
    • Place of Presentation
      苫小牧市
    • Year and Date
      2010-03-25
  • [Presentation] キメラべん毛モーターの温度応答トルクステップ解析2010

    • Author(s)
      井上裕一
    • Organizer
      べん毛研究交流会
    • Place of Presentation
      愛知県蒲郡市
    • Year and Date
      2010-03-16
  • [Presentation] Torque response of bacterial flagellar motor induced by transient heating2009

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      生物物理学会第47回年会
    • Place of Presentation
      徳島市
    • Year and Date
      2009-11-01
  • [Presentation] Torque response of bacterial flagellar motor induced by reversible temperature change2009

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
    • Place of Presentation
      京都市
    • Year and Date
      2009-09-08
  • [Presentation] Torque response of bacterial flagellar motor induced by reversible temperature change2009

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      International Symposium of post-silicon materials and devices research alliance project
    • Place of Presentation
      吹田市
    • Year and Date
      2009-09-05
  • [Presentation] 生体モーター運動活性の局所的熱変調2009

    • Author(s)
      井上裕一, 福岡創, 石島秋彦
    • Organizer
      特定領域研究膜超分子モーターの革新的ナノサイエンス第4回班会議
    • Place of Presentation
      沖縄県名護市
    • Year and Date
      2009-06-19
  • [Presentation] Torque response of the Na+-driven chimeric flagellar motor in E. coli induced by reversible temperature change2009

    • Author(s)
      Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
    • Organizer
      特定領域研究マルチスケール操作によるシステム細胞工学第7回公開シンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2009-03-06
  • [Presentation] Temperature-dependence of torque generation of the Na+-driven chimeric flagellar motor and visualization of the stator proteins in E. coli2009

    • Author(s)
      Akihiko Ishijima, Yuichi Inoue, Hajime Fukuoka
    • Organizer
      Biophysical Society 53rd Annual Meeting
    • Place of Presentation
      U. S. A., boston
    • Year and Date
      2009-03-04
  • [Presentation] Torque response of the sodium-driven chimeric flagellar motor in E. coli induced by reversible temperature change2009

    • Author(s)
      Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
    • Organizer
      BLAST X (Bacterial Locomotion and Signal Transduction X)
    • Place of Presentation
      Cuernavaca, Mexico
    • Year and Date
      2009-01-18
  • [Presentation] 温度変化によるキメラべん毛モーターの回転トルク応答2009

    • Author(s)
      井上裕一, 武田訓昌, 福岡創, 高橋泰人, 石島秋彦
    • Organizer
      2009年生体運動研究合同班会議
    • Place of Presentation
      東京
    • Year and Date
      2009-01-11
  • [Presentation] ナトリウム駆動型キメラべん毛モーターの温度依存性, 「ポストシリコン物質・デバイス創製基盤技術アライアンス」2008

    • Author(s)
      井上裕一, 武田訓昌, 福岡創, 高橋泰人, 石島秋彦
    • Organizer
      平成20年度成果報告会
    • Place of Presentation
      札幌市
    • Year and Date
      2008-12-25
  • [Presentation] Torque response of the Na+-driven chimeric flagellar motor in E. coli induced by reversible temperature change2008

    • Author(s)
      Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      博多市
    • Year and Date
      2008-12-05
  • [Presentation] Torque response of the Na^+-driven chimeric motor induced by reversible temperature change2008

    • Author(s)
      Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      The Fourth Workshop of the UK-Japan Bionanotechnology Collaboration
    • Place of Presentation
      神戸市
    • Year and Date
      2008-09-17
  • [Presentation] ナトリウム駆動型キメラべん毛モーターの温度依存性2008

    • Author(s)
      井上裕一
    • Organizer
      生物物理学会・東北支部会シンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2008-08-30
  • [Presentation] ナトリウム駆動型キメラべん毛モーター変異体の運動性2008

    • Author(s)
      井上裕一, Chien-Jung Lo, 福岡創, 高橋泰人, 曽和義幸, 本間道夫, Richard M. Berry, 石島秋彦
    • Organizer
      第5回東北大バイオサイエンスシンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2008-05-19
  • [Book] 現代界面コロイド科学の辞典, GFPの基本的原理とその発展系2010

    • Author(s)
      石島秋彦, 井上裕一, 福岡創
    • Total Pages
      120-121
    • Publisher
      丸善社
  • [Book] ナトリウム駆動型キメラべん毛モーターのトルク-スピード関係2009

    • Author(s)
      井上裕一, 福岡創, 石島秋彦
    • Total Pages
      292-293
    • Publisher
      生物物理
  • [Book] ナノイメージング第5章2008

    • Author(s)
      石島秋彦, 井上裕一, 福岡創, 田中裕人
    • Publisher
      エヌ・ティエス社
  • [Book] 実用精密位置決め技術事典2008

    • Author(s)
      石島秋彦, 井上裕一, 福岡創, 田中裕人, 曽和義幸
    • Total Pages
      639-645
    • Publisher
      産業技術サービスセンター
  • [Remarks]

    • URL

      http://www.tagen.tohoku.ac.jp/labo/ishijima/paper.html

URL: 

Published: 2013-07-31  

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