IMAGING ANALYSIS OF CELL SURFACE P2X4 RECEPTOR UP-REGULATED BY FLOW SHEAR STRESS

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: KOBAYASHI Takeshi Nagoya University, 医学系研究科, 助教 (40402565)
• Project Period (FY): 2008 – 2010
• Keywords: 血流 / P2X4 / 細胞内輸送 / イメージング / 機械刺激感知

Research Abstract

P2X4 receptor, a non-selective ATP-operated cation channel, in vascular endothelial cells (ECs) mediates flow-induced Ca^<2+> influx and subsequent NO production to regulate vascular tone. Although P2X4 is thought to be activated by the ATP released into the extracellular space from ECs by shear stress, our fluorescence microscopic observation in ECs without shear stress showed that majority of P2X4 molecules is localized on the intracellular vesicles. In this study we found that the expression level of cell surface P2X4 of ECs is up-regulated by shear stress, leading to an apparent increase in the sensitivity to external ATP.

Research Products

• [Journal Article] Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions. (2010)
  • Author(s): Kobayashi T, Sokabe M
  • Journal Title: Curr Opin Cell Biol. 22 Pages: 669-676
  • Peer Reviewed
• [Journal Article] Stem cell factor/KIT signaling plays a pivotal role in regulating pigmentation in mammalian hair (2009)
  • Author(s): Hachiya A, Sriwiriyanont P, Kobayashi T, Nagasawa A, Yoshida H, Ohuchi A, Kitahara T, Visscher MO, Takema Y, Tsuboi R, Boissy RE
  • Journal Title: J.Pathol. 218 Pages: 30-39
  • Peer Reviewed
• [Journal Article] Enzymological analysis of mutant protein kinase Cγ causing spinocerebellar ataxia type 14 and dysfunction in Ca^<2+> homeostasis. (2008)
  • Author(s): Adachi N, Kobayashi T, Takahashi H, Kawasaki T, Shirai Y, Ueyama T, Matsuda T, Seki T, Sakai N, Saito N
  • Journal Title: J.Biol.Chem. 283 Pages: 19854-19863
  • Peer Reviewed
• [Presentation] 細胞-基質接着部位におけるメカノトランスダクション:受動力覚から能動力覚へ 機械的ストレスの感知と疾患 (2010)
  • Author(s): 曽我部正博、早川公英、小林剛、辰巳仁史
  • Organizer: BMB2010(第83回日本生化学会大会・第33回日本分子生物学会年会 合同大会 ワークショップ1W6)
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] Single molecule imaging of signaling molecules and ion channels in living cells. (2009)
  • Author(s): Kobayashi T, Sokabe M
  • Organizer: The 36th International Congress of Physiological Science, Satellite Symposium #8 : Cardiac Electro-mechanical function, Cell-Organ Cross-Talk Revealed via Integration of Experiments and Models.
  • Place of Presentation: Naoshima, Kagawa
  • Year and Date: 20090802-20090804
• [Presentation] Flow-induced up-regulation of cell surface P2X4 channels. (2008)
  • Author(s): 小林剛、Fernando Lopez-Redondo、武田美江、田中瑞奈、古家喜四夫、山本希美子、安藤譲二、曽我部正博
  • Organizer: 第BMB2008(第81回日本生化学会大会・第31回日本分子生物学会年会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Presentation] 流れ刺激により誘導されるP2X4チャネルの細胞膜への移行 (2008)
  • Author(s): 小林剛、Fernando Lopez-Redondo、武田美江、田中瑞奈、古家喜四夫、山本希美子、安藤譲二、曽我部正博
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20081203-20081205
• [Book] ベーシックマスター細胞生物学(「細胞膜」神谷律、尾張部克志 編集 第5章) (2009)
  • Author(s): 小林剛
  • Total Pages: 90-111
  • Publisher: オーム社
• [Book] 組織細胞化学2008(全反射蛍光顕微鏡(TIRFM)を用いたイメージング) (2008)
  • Author(s): 小林剛
  • Total Pages: 137-147
  • Publisher: 日本組織細胞化学会
• [Remarks] ホームページ等
  • URL: http://www.med.nagoya-u.ac.jp/physiol2