2010 Fiscal Year Final Research Report
Investigation of the mechanism by which novel phosphorylated RNA polymerase II-interacting factors coordinate processes required for gene expression
| Project/Area Number |
20570162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Toyama |
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Principal Investigator |
HIROSE Yutaka University of Toyama, 大学院・医学薬学研究部(薬学), 准教授 (00218851)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子発現調節 / 転写 / RNAポリメラーゼII / リン酸化 / WWドメイン |
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| Research Abstract |
RNA polymerase II (Pol II) copies genetic information from DNA to RNA. The carboxy-terminal domain (CTD) of Pol II is subjected to reversible phosphorylation during transcription cycle and plays an important role in gene expression. Recently, I identified a novel human protein PCIF1 as a phosphorylated CTD-interacting protein. In this study, I investigated PCIF1's function in gene expression. My data suggests that PCIF1 binds Pol II at promoter region during early phase of transcription cycle and modulates phosphorylation level of the CTD.
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