2010 Fiscal Year Final Research Report
RNA damages and RNA quality control
| Project/Area Number |
20570168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
HAYAKAWA Hiroshi Fukuoka Dental College, 歯学部, 教授 (70150422)
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| Co-Investigator(Kenkyū-buntansha) |
ITOU Riyoko 福岡歯科大学, 歯学部, 講師 (10140865)
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| Project Period (FY) |
2008 – 2010
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| Keywords | RNA |
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| Research Abstract |
We searched for proteins that specifically bind to 8-oxoguanine-containing RNA from human HeLa cell extracts, and the candidate proteins were identified using mass spectrometry. Among the identified candidates, splicing isoform 1 of heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) and splicing isoform C1 of heterogeneous nuclear ribonucleoprotein C1/C2 (HNRNPC) exhibited strong abilities to bind to oxidized RNA. The amount of HNRNPD protein rapidly decreased when cells were exposed to hydrogen peroxide, an agent that enhances oxidative stress. Moreover, the suppression of HNRNPD expression by siRNA caused cells to exhibit an increased sensitivity to hydrogen peroxide. The application of siRNA against HNRNPC also caused an increase in sensitivity to hydrogen peroxide. Since no additive effect was observed with a combined addition of siRNAs for HNRNPD and HNRNPC, we concluded that the two proteins may function in the same mechanism for the accurate gene expression.
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