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2010 Fiscal Year Final Research Report

Histone deacetylation related with centromere functions

Research Project

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Project/Area Number 20570169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionNational Institute of Genetics

Principal Investigator

YAMAO Fumiaki  National Institute of Genetics, 分子遺伝研究系, 教授 (10158074)

Co-Investigator(Kenkyū-buntansha) TSUTSUI Yasuhiro  東京工業大学, 大学院・生命理工学研究科, 助教 (00390625)
Project Period (FY) 2008 – 2010
Keywords染色体機能 / セントロメア / 翻訳後修飾 / 染色体複製
Research Abstract

Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains : the central CENP-A(Cnp1) kinetochore domain and flanking heterochromatin domains. We showed that fission yeast Mcl1, a DNA polymerase alpha (Pol alpha) accessory protein, is critical for maintenance of centromeric chromatin. In a screen for mutants that alleviate both central domain and outer repeat silencing, we isolated several cos mutants, of which cos1 is allelic to mcl1. The mcl1-101 mutation caused reduced CENP-A(Cnp1) in the central domain and an aberrant increase in histone acetylation in both domains. These phenotypes were also observed in a mutant of swi7(+), which encodes a catalytic subunit of Pol alpha. Mcl1 formed S-phase-specific nuclear foci, which colocalized with those of PCNA and Pol alpha. These results suggest that Mcl1 and Pol alpha are required for propagation of centromere chromatin structures during DNA replication.

  • Research Products

    (9 results)

All 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results)

  • [Journal Article] A DNA Polymerase a Accessory Protein, Mcl1, Is Requiredfor Propagation of Centromere Structures in FissionYeast.2008

    • Author(s)
      T.Natsume, Y.Tsutsui, T.Sutani, E.M.Dunleavy, A.L.Pidoux, H.Iwasaki, K.Shirahige, R.C.Allshire, F.Yamao
    • Journal Title

      Plos One. 3(5)

      Pages: e2221

    • Peer Reviewed
  • [Journal Article] Mus81 is essential for sister chromatid recombination at broken replication forks.2008

    • Author(s)
      Y..Yamada, Y.Tsutsui, P.Russell, H.Iwasaki, B.Arcangioli.
    • Journal Title

      The EMBO J. 27

      Pages: 1378-1387

    • Peer Reviewed
  • [Presentation] Characterization for sumoylation of Schizosaccharomyses pombe Rad22 protein involved in homologous recombination and recombination repair2010

    • Author(s)
      Yumiko Kurokawa, Yasuhiro Tsutsui, Hiroshi Iwasaki, Fumiaki Yamao
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      20101208-20101212
  • [Presentation] Biochemical analysis of fission yeast F-box DNA helicase involved in regulation of homologous recombination2009

    • Author(s)
      Yasuhiro Tsutsui, Yumiko Kurokawa, Hiroshi Iwasaki, Fumiaki Yamao
    • Organizer
      第32回分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      20091210-20091214
  • [Presentation] Fission yeast Rhp51 is ubiquitinylated by F-box DNA helicase in vitro2009

    • Author(s)
      Yasuhiro Tsutsui, Yumiko Kurokawa, Hiroshi Iwasaki, Fumiaki Yamao
    • Organizer
      The International Symposium on "Chromosome cycle and genome dynamics"
    • Place of Presentation
      Nasu, Tochigi, Japan
    • Year and Date
      20091020-20091022
  • [Presentation] Fission yeast Mcl1, a DNA polymerase α accessory protein, interacts with SHREC complex in vivo2009

    • Author(s)
      Yasuhiro Tsutsui, Toyoaki Natsume, Takashi Sutani, Hiroshi Iwasaki, Katsuhiko Shirahige, Fumiaki Yamao
    • Organizer
      The International Symposium on "Chromosome cycle and genome dynamics"
    • Place of Presentation
      Nasu, Tochigi, Japan
    • Year and Date
      20091020-20091022
  • [Presentation] Characterization for in vitro sumonylation of S.bombe Rad22 protein involved in homologous recombination recombination repair.2009

    • Author(s)
      Yumiko Kurokawa, Yasuhiro Tsutsui, Hiroshi Iwasaki, Fumiaki Yamao
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      20091010-20091014
  • [Presentation] BIOCHEMICAL CHARACTERIZATION OF FISSION YEAST FBH1, A DNA HELICASE WITH F-BOX INVOLVED IN HOMOLOGOUS RECOMBINATION2009

    • Author(s)
      Yasuhiro Tsutsui, Yumiko Kurokawa, Hiroshi Iwasaki, Fumiaki Yamao
    • Organizer
      The 5th International Fission Yeast Meeting
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20090510-20090514
  • [Presentation] Characterization of fission yeast Mcl1 involved in propagation of centromere structure2008

    • Author(s)
      Y.Tsutsui, T.Natsume, F.Yamao
    • Organizer
      BMB2008
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      20081209-20081212

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Published: 2012-01-26   Modified: 2016-04-21  

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