Activation mechanism of pre-replication complex in eukaryote

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: IZUMI Masako The Institute of Physical and Chemical Research, 生物照射チーム, 専任研究員 (00280719)
• Project Period (FY): 2008 – 2010
• Keywords: 染色体複製 / Mcm10 / Mcm2-7

Research Abstract

Depletion of Mcm10 by siRNA inhibited origin firing, not fork progression. Using truncated mutants, I found Mcm10 interacted with Mcm2-7 through the intrinsically disorder domain. In addition, the genetical analyses showed that the conserved domain was essential for DNA replication. On the contrary, the Mcm2-7-interacting domain contributed to the retention of Mcm10 onto chromatin.

Research Products

• [Journal Article] The effects of trichostatin A on DNA damage response after X- ray irradiation in human cells. (2010)
  • Author(s): Izumi, M.
  • Journal Title: RIKEN Accel.Prog.Rep. 43 Pages: 275
• [Journal Article] Effect of X-ray irradiation on the expression of DNA-repair-related genes in rice. (2010)
  • Author(s): Shibukawa, T., Hayashi, Y., Hirano, T., Kazama, M., Izumi, M., Abe, T.
  • Journal Title: RIKEN Accel.Prog.Rep. 43 Pages: 276
• [Journal Article] Cell-killing effect of low dose of high-LET heavy ions (II). (2010)
  • Author(s): Tomita, M., Tsukada, T., Izumi, M.
  • Journal Title: RIKEN Accel.Prog.Rep. 43 Pages: 280
• [Journal Article] Trichostatin A facilitates the phosphorylation of histone H2AX after X-ray irradiation in normal human fibroblast. (2009)
  • Author(s): Izumi, M.
  • Journal Title: RIKEN Accel.Prog.Rep. 42 Pages: 275
• [Journal Article] Cell-killing effect of low dose of high-LET heavy ions (II). (2009)
  • Author(s): Tomita, M., Tsukada, T., Izumi, M.
  • Journal Title: RIKEN Accel.Prog.Rep. 42 Pages: 276
• [Journal Article] Gamma-H2AX foci formation after argon beam irradiation to the cultured animal cell. (2009)
  • Author(s): Takagi, K., Tsukada, T., Izumi, M., Kazama, Y., Hayashi, Y., Abe, T.
  • Journal Title: RIKEN Accel.Prog.Rep. 42 Pages: 274
• [Journal Article] DNA damage response after heavy-ion irradiation in mammalian quiescent cells. (2008)
  • Author(s): Izumi, M., Tsukada, T.
  • Journal Title: RIKEN Accel.Prog.Rep. 41 Pages: 219
• [Journal Article] Cell-killing effect of low dose of high-LET heavy ions. (2008)
  • Author(s): Tomita, M., Tsukada, T., Izumi, M.
  • Journal Title: RIKEN Accel.Prog.Rep. 41 Pages: 220
• [Journal Article] Antiarrhythmic effect of single heave-ion irradiation on diseased canine heart. (2008)
  • Author(s): Yoshioka, K., Amino, M., Furusawa, Y., Izumi, M., Tsukada, T.
  • Journal Title: RIKEN Accel.Prog.Rep. 41 Pages: 222
• [Journal Article] Enhancement of radiosensitivity by trichostatin A (TSA) after heavy-ion irradiation in human cancer cells.
  • Author(s): Izumi, M., Tsukada, T.
  • Journal Title: RIKEN Accel.Prog.Rep. (in press)
• [Presentation] ヒトMcm10の機能ドメインの解析 (2010)
  • Author(s): 泉雅子、柳憲一郎、水野武、杉村和人、今本尚子、奥村克純、花岡文雄
  • Organizer: 第33回分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Presentation] Analysis on the functional domains of Mcm10 in mammalian cells (2009)
  • Author(s): 泉雅子、柳憲一郎、水野武、杉村和人、今本尚子、奥村克純、花岡文雄
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 複製開始反応におけるヒトMcm10の機能解析 (2008)
  • Author(s): 泉雅子、柳憲一郎、水野武、今本尚子、花岡文雄
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Remarks] ホームページ
  • URL: http://www.nishina.riken.go.jp/newcontents/contents/about/research_group.html