2010 Fiscal Year Final Research Report
Functional analysis of transcriptional repressor RP58 in corticogenesis
| Project/Area Number |
20570172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
MARUYAMA Chiaki Tokyo Metropolitan Organization for Medical Research, 東京都神経科学総合研究所, 主席研究員 (00281626)
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| Co-Investigator(Kenkyū-buntansha) |
OKADO Haruo 財団法人東京都医学研究機構, 東京都神経科学総合研究所, 副参事研究員 (60221842)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 大脳皮質 / RP58 / 転写抑制因子 / 胚発生 / 神経細胞移動 / Ngn2 |
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| Research Abstract |
RP58, a transcriptional repressor, is expressed strongly in the developing neocortex and plays an essential role for corticogenesis. From the RP58-KO mice analysis, it is suggested that RP58 is important for neuronal differentiation, survival and migration. In the present study, in order to reveal the molecular mechanism for corticogenesis, RP58 downstream effecter genes and detailed migration defects have been analyzed. As a result, proneural bHLH factor, Ngn2 has been identified as RP58 target gene. Ngn2 activates RP58 transcription first, then induced RP58 represses Ngn2 transcription. This negative feedback mechanism between Ngn2 and RP58 allows transient expression of Ngn2. Using in utero electroporation, it was revealed that RP58 deficient cells can not convert from multipolar to bipolar and enter to locomotion mode in the cortical plate.
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[Journal Article] The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex2009
Author(s)
Okado H., Ohtaka-Maruyama C.,Sugitani Y.,Fukuda Y., Ishida R., Hirai S., Miwa A., Takahashi A., Aoki K., Mochida K., Suzuki O., Honda T., Nakajma K., Ogawa M.,Terashima T., Matsuda J., Kawano H., Kasai M.
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Journal Title
Dev Biol 331(2)
Pages: 140-151
Peer Reviewed
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[Journal Article] A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP582009
Author(s)
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H.
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Journal Title
Dev Cell. 6
Pages: 836-48
Peer Reviewed
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