Mechanisms of genomic stability maintenance by replication scaffold protein, AND-1

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570173
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: YOSHIZAWA Naoko (須賀田 直子) Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (30344071)
• Project Period (FY): 2008 – 2010
• Keywords: DNA複製 / 複製チェックポイント / 複製フォーク / 複製ストレス / DNA修復 / ゲノム不安定性 / 姉妹染色体交換

Research Abstract

Coordinated execution of DNA replication, checkpoint activation and postreplicative chromatid cohesion is intimately related to the replication fork machinery. Human AND-1 is localized at the replication scaffold, and is required for efficient DNA synthesis and its depletion increases DNA damage, delays progression of S phase, leads to cell death in cancer cells. AND-1 is also required for checkpoint activation in replication stress, inhibition of UV-RDS and homologous recombinational repair. This study indicates that AND-1 coordinates multiple cellular events in S phase and G2 phase.

Research Products

• [Journal Article] Eukaryotic DNA Replication : where, when and how? (2010)
  • Author(s): Hisao Masai, Seiji Matsumoto, Zhiying You, Naoko Yoshizawa-Sugata, Masako Oda
  • Journal Title: Annual Review of Biochemistry 79 Pages: 89-130
  • Peer Reviewed
• [Journal Article] Roles of human AND-1 in chromosome transactions in S phase. (2009)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Journal Title: J. Biol. Chem. 284(31) Pages: 20718-20728
  • Peer Reviewed
• [Presentation] Regulation of replication program and maintenance of genetic integrity (2010)
  • Author(s): Hisao Masai, Satoshi Yamazaki, Motoshi Hayano, Yutaka Kanoh, Seiji Matsumoto, Naoko Kakusho, Ai, Ishii, Michie Shimmoto, Masako Oda, Naoko Yoshizawa, Sayuri Itoh, Satomi Kudo
  • Organizer: 第33回日本分子生物学会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド(口頭発表)
  • Year and Date: 2010-12-10
• [Presentation] Regulation of origin firing program and checkpoint by Cdc7, Mrc1 and Rif1 (2010)
  • Author(s): Hisao Masai, Seiji Matsumoto, Motoshi Hayano, Yutaka Kanoh, Satoshi Yamazaki, Satomi Kudo, Naoko Kakusho, Rino Fukatsu, Naoko Yoshizawa, Ai, Ishii
  • Organizer: 7th 3R Symposium
  • Place of Presentation: Toyama conference center, Toyama(口頭発表)
  • Year and Date: 2010-10-29
• [Presentation] Roles of human replisome component AND-1 and Tim in S phase chromosome transaction (2010)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: 7th 3R Symposium (ポスター発表)
  • Place of Presentation: Toyama conference center, Toyama
  • Year and Date: 2010-10-28
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2009)
  • Author(s): Naoko Yoshizawa, Hisao Masai
  • Organizer: 第32回日本分子生物学会年会(口頭・ポスター発表)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20091209-20091212
• [Presentation] Regulation of replication fork integrity during S phase (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Mika Yokoyama, Michiyo Shimmoto, Naoko Kakusho, Naoko Yoshizawa, Taku Tanaka
  • Organizer: 第31回日本分子生物学会(BMB2008)(シンポジウム口頭発表)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20081209-20081212
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombinational repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: 6th 3R Symposium (ポスター発表)
  • Place of Presentation: Tsumagoi, Shizuoka
  • Year and Date: 20081027-20081030
• [Presentation] Regulation of replication fork in maintenance of genomic integrity (2008)
  • Author(s): Hisao Masai, Motoshi Hayano, Seiji Matsumoto, Yutaka Kanoh, Michie Shimmoto, Mika Yokoyama, Taku Tanaka, Naoko Kakusho, Naoko Yoshizawa, You Zhiying, Sayuri Ito, Chika Taniyama, Ai Ishii
  • Organizer: 6th 3R Symposium
  • Place of Presentation: Tsumagoi, Shizuoka(招待講演)
  • Year and Date: 20081027-20081030
• [Presentation] Human AND-1 is required for efficient DNA replication, checkpoint activation and homologous recombination repair (2008)
  • Author(s): Naoko Yoshizawa-Sugata, Hisao Masai
  • Organizer: EMBO Conference on Replication and Segregation of Chromosomes (ポスター発表)
  • Place of Presentation: Geilo, Norway
  • Year and Date: 20080616-20080620
• [Remarks] ホームページ
  • URL: http://rinshoken.igakuken.or.jp/