2010 Fiscal Year Final Research Report
Global identification and functional analysis of protein kinase substrates by a phosphoproteomic approach
| Project/Area Number |
20570177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KOSAKO Hidetaka The University of Tokushima, 疾患酵素学研究センター, 准教授 (10291171)
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| Project Period (FY) |
2008 – 2010
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| Keywords | プロテオーム / タンパク質キナーゼ / リン酸化 / MAPキナーゼ / 核膜孔複合体 / 核-細胞質間輸送 / 抗リン酸化抗体 |
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| Research Abstract |
A multi-step method for detecting proteins phosphorylated by ERK MAP kinase reveals many new ERK substrates, including three nucleoporins. Nup50 is phosphorylated in FG repeats by ERK in vitro and in vivo, suggesting a new mechanism by which MAP kinase signaling controls nuclear translocation of proteins.
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[Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport.2009
Author(s)
Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
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Journal Title
Nature Struct.Mol.Biol. 16
Pages: 1026-1035
Peer Reviewed
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[Journal Article] Phosphorylation of Nephrin triggers Ca^<2+> signaling by recruitment and activation of phospholipase C-γ1.2009
Author(s)
Harita, Y., Kurihara, H., Kosako, H., Tezuka, T., Sekine, T., Igarashi, T., Ohsawa, I., Ohta, S., Hattori, S.
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Journal Title
J.Biol.Chem. 284
Pages: 8951-8962
Peer Reviewed
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[Journal Article] Neph1, acomponent of the kidney slit diaphragm,is tyrosine phosphorylated by the Src family tyrosine kinase and modulates intracellular signaling by binding to Grb2.2008
Author(s)
Harita, Y., Kurihara, H., Kosako, H., Tezuka, T., Sekine, T., Igarashi, T., Hattori, S.
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Journal Title
J.Biol.Chem. 283
Pages: 9177-9186
Peer Reviewed
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