2010 Fiscal Year Final Research Report
Regulation of unequal cell division in animal oocyte meiosis
Project/Area Number |
20570180
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
TACHIBANA Kazunori Tokyo Institute of Technology, バイオ研究基盤支援総合センター, 准教授 (60212031)
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Project Period (FY) |
2008 – 2010
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Keywords | 不等分裂 / 減数分裂 / 卵母細胞 / 紡錘体 / 蛋白質キナーゼ |
Research Abstract |
During meiotic maturation of animal oocytes, two successive divisions occur without an intermediate phase of DNA replication, so that haploid gametes are produced. Moreover, these two divisions are extremely asymmetric, to ensure that most of the maternal stores are retained within the oocyte. This leads to the formation of daughter cells with different sizes : the large oocyte and the small polar bodies. It is not known what signal couples the spindle pole positioning to polar body formation. We have found that Mos-MAPK-Rsk kinase pathway is required for the normal polarbody extrusion. So, we approached the question by analyzing the proteins that are phosphorylated specifically by the kinase pathway during the polarbody extrusion. We have identified some candidates for the regulators for the spindle pole positioning. Now, we are checking each candidate play a role in the asymmetric cell division.
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Research Products
(10 results)
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[Journal Article] Initiation of DNA replication by fertilization is regulated by p90Rsk at pre-RC/pre-IC transition in starfish eggs.2010
Author(s)
Tachibana, K., Mori, M., Matsuhira, T., Karino, T., Inagaki, T., Nagayama, A., Nishiyama, A., Hara, M., Kishimoto, T.
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Journal Title
Proceedings of the National Academy of Sciences USA 16
Pages: 5006-5011
Peer Reviewed
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[Journal Article] Involvement of Mos-MEK-MAPK pathway in cytostatic factor (CSF) arrest in eggs of the parthenogenetic insect, Athalia rosae.2008
Author(s)
Yamamoto, D.S., Tachibana, K., Sumitani, M., Lee, J.M., Hatakeyama, M.
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Journal Title
Mechanisms of Development 125
Pages: 996-1008
Peer Reviewed
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