Genome-wide analysis of C.elegans JNK cascade

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: HISAMOTO Naoki Nagoya University, 理学研究科, 准教授 (80283456)
• Project Period (FY): 2008 – 2010
• Keywords: C.elegans / JNK / RNAi

Research Abstract

The JNK MAP kinase (MAPK) pathway plays a pivotal role in the various stress responses of evolutionarily diverse species. In C. elegans, a JNK-like MAPK pathway composed of MLK-1 MAPKKK, MEK-1 MAPKK and KGB-1 MAPK is known to act in a heavy metal stress response. However, the upstream or downstream components of this pathway remain unknown. It has been shown that the KGB-1 pathway is negatively regulated by VHP-1, a dual-specificity MAPK phosphatase, and that mutations defective in the KGB-1 pathway suppress the growth arrest caused by a loss-of-function mutation in the vhp-1 gene. Therefore, we performed genome-wide RNAi screening for vhp-1 suppressor to identify genes functioning in the KGB-1 pathway. As the result, we identified several genes including novel genes as vhp-1 suppressors. In addition, we identified an adaptor protein SHC-1, a protein kinase MAX-2 and a small G protein MIG-2 as upstream factors of MLK-1 and clarified their roles in KGB-1 cascade.

Research Products

• [Journal Article] elegans JIP3 protein UNC-16 functions as an adaptor to link kinesin-1 with cytoplasmic dynein. (2011)
  • Author(s): Arimoto M, Koushika SP, Choudhary B, Li C, Matsumoto K, Hisamoto N The C.
  • Journal Title: J.Neurosci. 31 Pages: 2216-2224.
• [Journal Article] Regulation of Anoxic Death in Caenorhabditis elegans by Mammalian Apoptosis Signal-Regulating Kinase (ASK) Family Proteins. (2011)
  • Author(s): Hayakawa T, Kato K, Hayakawa R, Hisamoto N, Matsumoto K, Takeda K, Ichijo H.
  • Journal Title: Genetics. 187 Pages: 785-792.
• [Journal Article] The ERK-MAPK pathway regulates longevity through SKN-1 and insulin-like signaling in Caenorhabditis elegans. (2010)
  • Author(s): Okuyama T, Inoue H, Ookuma S, Satoh T,Kano K, Honjoh S, Hisamoto N, Matsumoto K, Nishida E.
  • Journal Title: J.Biol.Chem. 285 Pages: 30274-30281.
• [Journal Article] Phosphorylation of the conserved transcription factor ATF-7 by PMK-1 p38 MAPK regulates innate immunity in Caenorhabditis elegans. (2010)
  • Author(s): Shivers RP, Pagano DJ, Kooistra T, Richardson CE, Reddy KC, Whitney JK, Kamanzi O, Matsumoto K, Hisamoto N, Kim DH.
  • Journal Title: PLoS Genet. 6 Pages: e1000892.
• [Journal Article] The Caenorhabditis elegans Ste20-related kinase and Rac-type small GTPase regulate the c-Jun N-terminal kinase signaling pathway mediating the stress response. (2010)
  • Author(s): Fujiki K, Mizuno T, Hisamoto N, Matsumoto K.
  • Journal Title: Mol.Cell.Biol. 30 Pages: 995-1003.
• [Journal Article] The germinal center kinase GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C.elegans germline. (2009)
  • Author(s): Schouest KR, Kurasawa Y, Furuta T, Hisamoto N, Matsumoto K, Schumacher JM.
  • Journal Title: PLoS One 4 Pages: e7450.
• [Journal Article] LRRK2 modulates vulnerability to mitochondrial dysfunction in Caenorhabditis elegans. (2009)
  • Author(s): Saha S, Guillily MD, Ferree A, Lanceta J, Chan D, Ghosh J, Hsu CH, Segal L, Raghavan K, Matsumoto K, Hisamoto N, Kuwahara T, Iwatsubo T, Moore L, Goldstein L, Cookson M, Wolozin B.
  • Journal Title: J.Neurosci. 29 Pages: 9210-9218.
• [Journal Article] Caenorhabditis elegans FOS-1 and JUN-1 regulate plc-1 expression in the spermatheca to control ovulation. (2009)
  • Author(s): Hiatt SM, Duren HM, Shyu YJ, Ellis RE, Hisamoto N, Matsumoto K, Kariya K, Kerppola TK, Hu CD.
  • Journal Title: Mol.Biol.Cell. 20 Pages: 3888-3895.
• [Remarks] ホームページ等