2010 Fiscal Year Final Research Report
Positive and negative factors responsible for inter-domain DNA transfer between bacteria and yeasts
| Project/Area Number |
20570221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MORIGUCHI Kazuki 広島大学, 大学院・理学研究科, 講師 (30294523)
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| Research Collaborator |
TANAKA Katsuyuki 株式会社建設環境研究所 (10363045)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 水平遺伝子伝達 / 生物界間接合 / T-DNA輸送 / 細菌 / アグロバクテリア / 酵母 / 真核生物 / 接合遺伝子 |
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| Research Abstract |
Agrobacterial T-DNA transfer and Trans-kingdom conjugation from Gram-negative bacteria to yeasts are interesting phenomena in that bacteria actively transfer DNA horizontally to eukaryotes. This study with a yeast as a model eukaryotic recipient revealed that yeast genes mainly for vATPase subunits are important for Trans-kingdom conjugation, and that yeast genes including RAD2 and SRS2 are important for the agrobacteria-mediated transformation. Each of the genes is likely to function in a process which had been specialized between the two phenomena. This study also developed useful tools for accurate but easy modification on huge-size plasmids and enabled highly efficient but easy transformation.
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Research Products
(25 results)
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[Journal Article] Noveltoxin-antitoxin system composed of serineprotease and AAA-ATPase homologuesdetermines the high stability andincompatibility of the tumor-inducing plasmidpTiC58.2009
Author(s)
Yamamoto, S., Kiyokawa, K., Tanaka, K., Moriguchi, K, , Suzuki, K.
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Journal Title
J. Bacteriol.
Volume: 191
Pages: 4656-4666
Peer Reviewed
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[Journal Article] A bility of Agrobacteriumtumefaciens and A. rhizogenes strains2009
Author(s)
K. Tanaka, H. H. Arafat, H. Urbanczyk, S. Yamamoto, K. Moriguchi, H. Sawada, and K. Suzuki
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Journal Title
inability of A. vitis and A. rubi strains to adapt tosalt-insufficient environment, and taxonomicsignificance of a simple salt requirement test inthe pathogenic Agrobacterium speciesJ. Gen. Appl. Microbiol.
Volume: 55
Pages: 35-41
Peer Reviewed
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