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2010 Fiscal Year Final Research Report

Establishment and utilization of simple and rapid soybean transformation system in a Japanese variety, Kariyutaka

Research Project

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Project/Area Number 20580003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHokkaido University

Principal Investigator

YAMADA Tetsuya  Hokkaido University, 大学院・農学研究院, 助教 (70374618)

Co-Investigator(Kenkyū-buntansha) KITAMURA Keisuke  北海道大学, 大学院・農学研究院, 教授 (50111240)
Project Period (FY) 2008 – 2010
Keywords形質転換 / 大豆 / アグロバクテリウム / 種子 / Myb転写因子 / フラボノイド
Research Abstract

An efficient and stable transformation technology in higher plants is used to identify the gene function or to develop new varieties. Soybean transformation has continued to be laborious and the frequency of recovered transgenic plants is still low. We found that a Japanese soybean variety, Kariyutaka, possessed advantageous characters for transformation. We developed a simple and rapid transformation system in Kariyutaka. The transformation frequency in the method presented in this study was 4 times as high as in that obtained with our previous method.

  • Research Products

    (7 results)

All 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean.2010

    • Author(s)
      T Yamada, S Watanabe, M Arai, K Harada, K Kitamura
    • Journal Title

      Plant Biotechnology 27

      Pages: 217-220

    • Peer Reviewed
  • [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1.2010

    • Author(s)
      Liu B, S Watanabe, T Uchimiya, F Kong, A Kanazawa, Z Xia, A Nagamatsu, M Arai, T Yamada, K Kitamura, Masuta C, K Harada, J Abe
    • Journal Title

      Plant Physiology 153

      Pages: 198-210

    • Peer Reviewed
  • [Presentation] 形質転換技術を利用したダイズ種子成分の代謝工学2011

    • Author(s)
      山田哲也
    • Organizer
      第5回ダイズ研究会(招待講演)
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-03-05
  • [Presentation] Establishment of stable transformation system mediated by Agrobacterium in Japanese soybean variety.2009

    • Author(s)
      山田哲也, 新井麻衣子, 松田史生, 斉藤和季, 喜多村啓介
    • Organizer
      World Soybean Conference Research VIII P.232
    • Place of Presentation
      中国北京
    • Year and Date
      20090810-20090815
  • [Presentation] アグロバクテリウムを介したダイズ形質転換系の確立とその利用2009

    • Author(s)
      山田哲也・松田史生・斉藤和季・新井麻衣子・渡辺啓史・原田久也・喜多村啓介
    • Organizer
      第27回日本植物細胞分子生物学会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-07-31
  • [Book] ダイズのゲノム科学と組換え育種の現状と展望、サイエンスフォーラム大豆のすべて(山田哲也担当)2010

    • Author(s)
      喜多村啓介編集
    • Total Pages
      67-71
  • [Remarks] ホームページ等

    • URL

      http://www.agr.hokudai.ac.jp/ikushu/idenshigen/

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Published: 2012-01-26   Modified: 2016-04-21  

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