Study of sel/non-selfrecognition system in sel/cross incompatibility of wild type and cultivated sweet potato

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Mie University
• Principal Investigator: TSUCHIYA Tohru Mie University, 生命科学研究支援センター, 准教授 (30293806)
• Project Period (FY): 2008 – 2010
• Keywords: 自家不和合性 / サツマイモ野生種 / Ipomoea trifida / 交配不和合性 / 自他認識Ipomoea batatas / S遺伝子

Research Abstract

Wild type sweet potato (Ipomoea trifida) has sporophytic self-incompatibility (SSI) system, but this system is not based on the SPII/SCR and SRK system, which is observed in Brassica. To identify S genes of Ipomoea SSI system, S-locus genomic regions from several S-haplotypes, including self-compatible (Sc) mutant, were isolated and analyzed. According to the shotgun sequencing of S-locus genomic regions from four S-haplotypes, the length of the S-locus genomic region was parallel to the dominant-recessive hierarchy of S-genes ; longer in dominants, shorter in recessives. In the Sc mutant line, all S-candidate genes ; AB2 for male, and SE2 and SEA for female ; were located in the Sc-S-locus, however, AB2 and SEA genes were duplicated, and two SEA genes indicated ectopic expression in the male reproductive organs. It is possible to consider that this ectopic expression of SEA genes make Sc line as self-compatible. S-candidate gene analyses of wild type (I.trifida) and cultivated (I.batatas) indicated that S_3 haplotype ofLtrifzda is conserved in the I.batatas. This result indicates that the self/cross-incompatibility in cultivated sweet potato is based on the same molecular mechanism as in the self-incompatibility of I.trifida.

Research Products

• [Journal Article] Family 17 and 28 Carbohydrate-Binding Modules Discriminate Different Cell Wall Sites in Sweet Potato Roots. (2010)
  • Author(s): Araki Y., Karita S., Tsuchiya T., Kondo M., Goto M.
  • Journal Title: Biosci.Biotechnol.Biochem. 74 Pages: 802-805
  • Peer Reviewed
• [Journal Article] Kawagishi-Kobayashi M.High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen Development. (2009)
  • Author(s): Endo M., Tsuchiya T., Hamada K., Kawamura S., Yano K., Ohshima M., Higashitani A., Watanabe M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1911-1922
  • Peer Reviewed
• [Journal Article] Bicknell R.Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella where two dominant loci function to enable apomixes.
  • Author(s): Koltunow A., Johnson S., Rodrigues J., Okada T., Yingkao H., Tsuchiya T., Wilson S., Fletcher P., Ito K., Suzuki G., Mukai Y., Fehrer J.
  • Journal Title: Plant J. in press
  • Peer Reviewed
• [Presentation] サツマイモ野生種(Ipomoea trifida)の自家不和合性におけるS遺伝子の同定に向けたアプローチ (2010)
  • Author(s): 土屋亨
  • Organizer: 基礎生物学研究所研究集会「アサガオ研究集会」
  • Place of Presentation: 基礎生物学研究所・岡崎コンファレンスセンター
  • Year and Date: 2010-11-13
• [Presentation] Sporophytic self-incompati-bility system in Ipomoea trifida, a close relative of sweet potato. (2010)
  • Author(s): 土屋亨
  • Organizer: International symposium of intercellular recognition and allogeneic authentication : perspectives of reproduction mechanisms shared by animals and plants.
  • Place of Presentation: サーウィンストンホテル・名古屋
  • Year and Date: 2010-01-13
• [Book] Self-Incompatibility in Flowering Plants-Evolution, Diversity and Mechanisms (Franklin-Tong VE.ed.)(Chapter 12 : Molecular Genetics of Sporophytic Self-Incompatibility in Ipomoea, a Member of the Convolvulaceae.) (2008)
  • Author(s): Kowyama Y., Tsuchiya T., Kakeda K.
  • Total Pages: 259-274
  • Publisher: Springer-Verlag, Berlin Heidelberg.
• [Book] Handbook of New Technologies for Genetic Improvement of Legumes (Kirti PB ed.)(Chapter 31 "Gene Expression Analysis with DNA Array Technology in Legumes") (2008)
  • Author(s): Watanabe M., Endo M., Hakozaki H., Masuko H., Park J-I., Kawagishi-Kobayashi M., Tsuchiya T.
  • Total Pages: 467-476
  • Publisher: CRC Press (Taylor & Francis Group).