2010 Fiscal Year Final Research Report
Integration of chromosome maps of allogamous diploid red clover.
| Project/Area Number |
20580006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kobe University |
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Principal Investigator |
OHMIDO Nobuko Kobe University, 人間発達環境学研究科, 教授 (30343263)
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| Project Period (FY) |
2008 – 2010
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| Keywords | マメ科植物 / 染色体地図 / 遺伝子地図 / FISH / 画像解析 |
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| Research Abstract |
Red clover (Trifolium pratense L.) is a forage legume, an allogamous diploid (2n=2x=14) with 440Mb genome size. Seven prometaphase chromosomes were identified by fluorescence in situ hybridization (FISH) using 26S and 5S rDNA probes and bacterial artificial chromosome (BAC) clones and chromosome image analysis system ver.4.0 (CHIAS4). 14 BAC clones located close to the both end of each linkage group (LG), representatively LG1-7, exclusively hybridized to the distal regions of Chromosome 4, 2, 6, 5, 1, 7 and 3, respectively. Chromosomes images of FISH mapping were quantitatively analyzed based on CHIAS4 and construct of quantitative idiogram. This is the first complete chromosome map of red clover and integration of the genetic linkage maps and the quantitative chromosome maps provides valuable information on the genetic data and insight into the legumes genetics. This study should contribute to provide significant data for DNA markers and molecular breeding in legume varieties.
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[Journal Article] Genome structure of the legume, Lotus japonicus.2008
Author(s)
S.Sato, Y.Nakamura, T.Kaneko, E.Asamizu, T.Kato, M.Nakao, S.Sasamoto, A.Watanabe, A.Ono, K.Kawashima, T.Fujishiro, M.Katoh, M.Kohara, Y.Kishida, C.Minami, S.Nakayama, N.Nakazaki, Y.Shimizu, S.Shinpo, C.Takahashi, T.Wada, M.Yamada, N.Ohmido, M.Hayashi, K.F
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Journal Title
DNA Res. 15
Pages: 227-239
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