2010 Fiscal Year Final Research Report
The visualization of amitosis cells and genetic analysis of somatic hybrids in Citrus species
| Project/Area Number |
20580034
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Horticulture/Landscape architecture
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
KUNITAKE Hisato University of Miyazaki, 農学部, 教授 (80289628)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | カンキツ / 細胞融合 / アミトーシス / 育種 / 染色体 |
|---|
| Research Abstract |
Tetraploid culture cells were induced successfully from the long term-subcultured embryogenic calli of Satsuma mandarin (Citrus unshiu Marc.), although it was not possible to observe the amitosis cells. Genetic analysis of the putative triploid somatic hybrid SG1 obtained from electrofusion between Shougun mandarin (C.reticulata Blanc., 2n=2x=18)and L-1 grapefruit (C.paradisi Macf., 2n=2x=18)were investigated. Results of flow cytometry and RAPD analyses suggested that this was a hybrid with the haploid genome of the shougun mandarin. This triploid somatic hybrid SG1 was also the grapefruit with excellent characteristics such as seedless and parthenocarpy.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation]2008
Author(s)
安田喜一, 國武久登, 中川匠子, 黒木宏憲, 八幡昌紀, 平田力也, 吉倉幸博, 川上郁夫, 杉本安寛
Organizer
平成20年度園芸学会秋季大会
Place of Presentation
三重大学
Year and Date
20080927-20080929
-
