2010 Fiscal Year Final Research Report
Analysis of AVR-Pia gene of trice blast fungus and its application for the pathogenicity differentiation
| Project/Area Number |
20580043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SONE Teruo Hokkaido University, 大学院・農学研究院, 准教授 (00333633)
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| Project Period (FY) |
2008 – 2010
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| Keywords | いもち病菌 / Magnaporthe oryzae / PCR / イネ / 非病原性遺伝子 / エフェクター / 突然変異 / 病原性 |
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| Research Abstract |
Successful molecular cloning and identification of AVR-Pia avirulence gene from Magnaporthe oryzae was demonstrated in this study. AVR-Pia gene was located at the same position as the locus which was genetically analyzed using Chinese isolates. AVR-Pia was expressed only during infection, and the product was found to be secreted into compatible host cells, through BICs of the infection hyphae. Mechanism of gene deletion in the mutant Ina168m95-1 was elucidated to be the homologous recombination between two adjacent copies of the DNA transposon Occan. PCR amplification of the gene is effective way to determine the pathogenicity toward rice cultivar with Pia R gene.
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[Journal Article] A multi-faceted genomics approach allows the isolation of rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes.2011
Author(s)
Y.Okuyama, H.Kanzaki, A.Abe, K.Yoshida, M.Tamiru, H.Saitoh, T.Fujibe, H.Matsumura, M.Shenton, D.C.Galam, J.Undan, A.Ito, T. Sone, R.Terauchi.
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Journal Title
Plant Cell 66
Pages: 467-479
Peer Reviewed
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[Journal Article] Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.2009
Author(s)
S.Miki, K.Matsui, H.Kito, K.Otsuka, T.Ashizawa, N.Yasuda, S.Fukiya, J.Sato, K.Hirayae, Y.Fujita, T.Nakajima, F.Tomita, T. Sone
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Journal Title
Mol.Plant Pathol. 10
Pages: 361-374
Peer Reviewed
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