2010 Fiscal Year Final Research Report
Molecular characterization of the TIA-1 like RNA binding protein of the silk worm, Bombyx mori.
| Project/Area Number |
20580051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
KOTANI Eiji Kyoto Institute of Technology, 工芸科学研究科, 准教授 (10273541)
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| Project Period (FY) |
2008 – 2010
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| Keywords | チョウ目昆虫 / NA結合蛋白質 / 転写産物制御 |
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| Research Abstract |
BmTRN-1 from the silkworm, Bombyx mori, is an RNA-binding protein homologous to mammalian TIA-1/TIAR, that possesses three RNA recognition motifs (RRMs) followed by a C-terminal auxiliary domain. Previous studies showed that the function of the BmTRN-1 is possibly involved in several mechanisms of RNA metabolism including the regulation of transcripts in the Bombyx cultured cells. In this study, the RRM2 and RRM1 domain of BmTRN-1 were found to be related to its nuclear export and nuclear accumulation, respectively. Also, Analyses by using the expressed GFP-BmTRN-1 fusion protein in the cells indicated that the BmTRN-1 distribution is drastically changed, and further indicated that the protein is distributed in the cytoplasm and forms aggregate structure, especially when the cells are infected by a baculovirus, Bombyx mori nucleopolyhedrovirus (BmNPV). Protein expression level of a BmNPV reporter protein was shown to be altered both by the BmTRN-1-overexpression and BmTRN-1-knockdown in the cultured cells and the transgenic individual tissues. In addition, we found that BmTRN-1 interacts with one of the BmNPV's nucleic acid-binding protein and the C-terminal auxiliary domain of BmTRN-1 is important for its interaction with the viral protein. Thus, it was indicated from these findings that the interaction between BmTRN-1 and the viral protein relates to the possible cellular function of resisting baculovirus proliferation.
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[Journal Article] Immobilization of protein kinase C into cypovirus polyhedra.2010
Author(s)
Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
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Journal Title
J.Insect Biotech. Sericol. 79
Pages: 15-20
Peer Reviewed
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[Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized-cytokines for mammalian cell culture.2009
Author(s)
Ijiri, H., Coulibaly, F., Nishimura, G., Nakai, D., Chiu, E., Takenaka, C., Ikeda, K., Nakazawa, H., Hamada, N., Kotani, E., Metcalf, P., Kawamata, S., Mori, H.
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Journal Title
Biomaterials 30
Pages: 4297-4308
Peer Reviewed
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[Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1 from the silkworm, Bombyx mori ; a study of it's overexpression and sub-cellular distribution during the baculovirus infection process.2009
Author(s)
Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
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Journal Title
J.Insect Biotech. Sericol. 78
Pages: 39-51
Peer Reviewed
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[Journal Article] Molecular Characterization of a TIA-1 like RNA-binding protein in the cells derived from the fall armyworm, Spodoptera frugiperda(Lepidoptera : Noctuidae).2009
Author(s)
Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E.
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Journal Title
Bioscience Biotechnology and Biochemistry 73
Pages: 648-656
Peer Reviewed
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