2010 Fiscal Year Final Research Report
Are rare earth elements essential for growth of methylotrophs?
| Project/Area Number |
20580075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
KAWAI Keiichi Gifu University, 応用生物科学部, 教授 (00002064)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMA Tomonori 岐阜大学, 応用生物科学部, 准教授 (60252128)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 希土類元素 / メチロトローフ / メタノール脱水素酵素 / Methylobacterium / Bradyrhizobium / xoxF / mxaF' / ランタン / セリウム |
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| Research Abstract |
Methanol dehydrogenase (MDH) of Methylobacterium sp.MAFF211642, a methyltrophic bacterium, was remarkably increased by addition of La to methanol medium, indicating that the MDH was induced by La.Induction of MDH by La was observed in Methylobacterium spp.MDH of Bradyrhizobium sp.MAFF 211645 was also induced by Ce.Thus, La or Ce can induce MDH in methylotrophic bacteria. Furthermore, molecular structure of both MDHs was homodimer that was different to the heterotetrameric structure of Ca-dependent MDH in Methylobacterium spp.In addition La-induced MDH in Methylobacterium spp.and Ce-induced MDH in Bradyrhizobium sp.MAFF211645 were found to be encoded by xoxF and mxaF', respectively. These phenomena were also observed in well-studied M.extorquens AM1.
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