2010 Fiscal Year Final Research Report
Study on the post transcriptional regulation of yeast gene expression under stressed conditions and brewing process
| Project/Area Number |
20580077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
IZAWA Shingo Kyoto University, 工芸科学研究科, 准教授 (10273517)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 出芽酵母 / エタノール / mRNA核外輸送 / ストレス / 醸造 |
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| Research Abstract |
We found that severe ethanol stress generates budding yeast stress granules (SGs) in a manner independent of the phosphorylation of eIF2alpha. The concentration that generated budding yeast SGs (above 10%) was higher than that causing P-bodies (more than 6%), and P-bodies were assembled prior to SGs. As well as mammalian SGs, the assembly of budding yeast SGs under ethanol stress was blocked by cycloheximide. On the other hand, the budding yeast SGs caused by ethanol stress contained eIF3c but not eIF3a and eIF3b, although the eIF3 complex is a core constituent of mammalian SGs. We also found that the formation of budding yeast SGs might play a role for sufficient recovery from ethanol stress. Additionally, we found novel phenomena about the selective export of mRNA and hyperadenylation of HSP mRNAs under ethanol stressed conditions and brewing process.
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