2010 Fiscal Year Final Research Report
Structure and function of streptococcal system for heparin degradation/import and its involvement in infectious diseases
| Project/Area Number |
20580078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
HASHIMOTO Wataru Kyoto University, 大学院・農学研究科, 准教授 (30273519)
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| Research Collaborator |
MURATA Kousaku 京都大学, 大学院・農学研究科, 教授 (90142299)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 連鎖球菌 / 宿主細胞外マトリックス / グリコサミノグリカン / 多糖リアーゼ / 不飽和グルクロニルヒドロラーゼ / 侵入・感染 |
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| Research Abstract |
Pathogenic streptococci produce unsaturated glucuronyl hydrolase (UGL) together with polysaccharide lyase responsible for completely degrading glycosaminoglycans, and the streptococcal UGLs prefer sulfated substrates. This suggests that the substrate specificity is feasible for bacterial infection through degradation of mammalian extracellular matrices with sulfate groups. The motif "R-//-Sxx(S)xK" in UGL is prerequisite for degradation of sulfated substrates.
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[Remarks] 2010年8月12日、「京都大学オープンキャンパス2010」の開催にあたり、本成果を含めた研究内容を高校生やその保護者に紹介した。
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[Remarks] 2010年9月14日文部科学省の「平成23年度科学・技術関係予算についてのパブリック・コメント」募集に際し、科学技術研究に関する意見を提出した。
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[Remarks] 2010年10月23、24日、「京都大学宇治キャンパス祭」にて、一般市民向けに研究内容を紹介するパンフレットとポスターの作成に協力した。
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[Remarks] Yusuke Nakamichi, Yukie Maruyama, Ryuichi Takase, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata. Loop movement over the active cleft in streptococcal unsaturated glucuronyl hydrolase. SPring-8 User Experiment Report, 2010A1279.
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[Remarks] Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata. Crystal structure of unsaturated glucuronyl hydrolase from Streptcoccus agalactiae. PDB ID, 3ANJ, 20100902
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[Remarks] Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata. Crystal structure of unsaturated glucuronyl hydrolase mutant D175Nfrom Streptcoccus agalactiae. PDB ID, 3ANI, 2010年9月2日.
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[Remarks] Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata. Crystal structure of unsaturated glucuronyl hydrolase mutant D175Nfrom Streptcoccus agalactiae complexed with dGlcA-GalNAc6S PDB ID, 3ANK, 20100902
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[Remarks] Yusuke Nakamichi, Yukie Maruyama, Takafumi Itoh, Akihito Ochiai, Kohei Ogura, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata. Crystal structure of streptococcal unsaturated glucuronyl hydrolase in complex with sulfated substrate. SPring-8 User Experiment Report, 2009B1201.
