Molecularbiological study on a novel organomercurial decomposing enzyme from iron-oxidizing bacterium Acidithiobacillus ferrooxidans and use of the enzyme for bioremediation of mercury

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Okayama University
• Principal Investigator: SUGIO Tsuyoshi Okayama University, 大学院・自然科学研究科, 教授 (20033269)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Fumiaki 岡山大学, 環境管理センター, 准教授 (90294446)
• Project Period (FY): 2008 – 2010
• Keywords: 鉄酸化細菌 / 水銀還元酵素 / 有機水銀分解酵素 / チトクロームcオキシダーゼ / Acidithiobacillus ferrooxidanas / iron-oxidizing bacterium / mercury / organomercurial

Research Abstract

It has been known that iron-oxidizing bacterium Acidithiobacillus ferrooxidans has a mercuric reductase (MerA) which reduces Hg^<2+> to a volatilizable metal mercury (Hg^0), but not has a mercuric lyase (MerB) which cleaves a C-Hg bond in organomercurials. Author newly found a novel Hg^0 volatilizing enzyme system in a highly mercury resistant A. ferrooxidans MON-1. Namely, strain MON-1 has a mercury resistant aa3-type cytochrome c oxidase ( aa_3) and α- and β-subunits in aa_3 has activities to cleave C-Hg bond in organomercurials and to reduce Hg^<2+> to Hg^0.

Research Products

• [Journal Article] Volatilization of metal mercury from organomercurials by highly mercuryresistant Acidithiobacillus ferrooxidans MON-1, Biosci. (2010)
  • Author(s): Sugio, T., Komoda, T., Okazaki, Y., Takeda, Y., Nakamura, S., Takeuchi, F.
  • Journal Title: Biotechnol. Biochem. Vol.74 Pages: 1007-1012
  • Peer Reviewed
• [Journal Article] Sulfite oxidation catalyzed by aa3-type cytochrome c oxidase in Acidithiobacillus ferrooxidans. (2010)
  • Author(s): Sugio, T, Ako, A., Takeuchi, F.
  • Journal Title: Biosci. Biotechnol. Biochem. Vol.74 Pages: 2242-2247
  • Peer Reviewed
• [Journal Article] Existence of ferrous irondependent mercury reducing enzyme system in sulfur-grown A. ferrooxidans MON-1 cells. (2009)
  • Author(s): Sugio, T., Taha, T.M., Negishi, A., Takeuchi, F.
  • Journal Title: Adv. Materials. Research. Vol.71-73 Pages: 745-748
  • Peer Reviewed
• [Journal Article] Ferrous iron production mediated by tetr athionate hydrolase in tetrathionate-, sulfur-, and iron-grown Acidithiobacillus ferrooxidans ATCC 23270. (2009)
  • Author(s): Sugio, T., Taha, T.M., Takeuchi, F.
  • Journal Title: Biosci. Biotech. Biochem. Vol.71 Pages: 2663-2669
  • Peer Reviewed
• [Journal Article] Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithio- bacillus ferrooxidans ATCC 23270 cells. (2009)
  • Author(s): Taha, T.M., Takeuchi, F., Sugio, T.
  • Journal Title: Adv. Materials Research. Vol.71-73 Pages: 243-246
  • Peer Reviewed
• [Journal Article] Isolation and characterization of Acidithiobacillus ferrooxidans strain D3-2 active in copper bioleaching from a copper mine in Chile. (2008)
  • Author(s): Sugio, T., Wakabayashi, M., Kanao, T., Takeuchi, F.
  • Journal Title: Biosci. Biotechnol. Biochem. Vol.72 Pages: 998-1004
  • Peer Reviewed
• [Journal Article] Reduction of Hg^<2+> with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1. (2008)
  • Author(s): Sugio, T., Fujii, M., Ninomiya, Y., Kanao, T., Negishi, A., Takeuchi, F.
  • Journal Title: Biosci. Biotechnol. Biochem. Vol.72 Pages: 1756-1763
  • Peer Reviewed
• [Journal Article] Reconstitution of iron oxidase from sulfur-grown Acidithio- bacillus ferrooxidans. (2008)
  • Author(s): Taha, T.M., Kanao, T., Takeuchi, F., Sugio, T.
  • Journal Title: Appl. Environ. Microbiol. Vol.74 Pages: 6808-6810
  • Peer Reviewed
• [Presentation] 高度水銀耐性鉄酸化細菌Acidithiobacillus ferrooxidans MON-1株由来aa_3型cytochrome c oxidase subunitsによる2価水銀の金属水銀への還元 (2010)
  • Author(s): 杉尾剛, 竹田有希、竹内文章
  • Organizer: 日本生物工学会2010年度大会
  • Place of Presentation: シーガイア(宮崎市)
  • Year and Date: 2010-10-29
• [Presentation] 鉄酸化細菌A. ferrooxidansの外膜におけるaa_3型cytochrome c oxidaseの存在 (2010)
  • Author(s): 狩野典香, 竹内文章, 杉尾剛
  • Organizer: 日本農芸化学会2010年度中四国支部大会
  • Place of Presentation: 香川大学農学部(高松市)
  • Year and Date: 2010-09-25
• [Presentation] 銅のバイオリーチング活性の高い鉄酸化細菌A.ferrooxidans D3-2株は亜硫酸耐性である (2010)
  • Author(s): 小野田桂子、竹内文章、杉尾剛
  • Organizer: 日本農芸化学会2010年度中四国支部大会
  • Place of Presentation: 香川大学農学部(高松市)
  • Year and Date: 2010-09-25
• [Presentation] Mercury Volatilization from Organomercurials by Mercury-Resistant Acidithiobacillus ferrooxidans MON-1 (2010)
  • Author(s): SUGIO T., KOMODA, T., OKAZAKI, Y., TAKEDA Y., NAKAMUR, S., TAKEUCHI F.
  • Organizer: アメリカ微生物学会2010年度大会
  • Place of Presentation: コンベンションセンタ(サンジエゴ、USA)
  • Year and Date: 2010-05-26
• [Presentation] 高度水銀耐性鉄酸化細菌Acidithiobacillus ferrooxidans MON-1株由来aa_3型cytochrome c oxidaseによる有機水銀の分解 (2009)
  • Author(s): 杉尾剛, 薦田友恵、岡崎夕子、竹田有希、T.M.Taha, 竹内文章
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋市(名古屋大学工学部)
  • Year and Date: 2009-09-24
• [Presentation] Existence of ferrousiron-dependent mercury reducing enzyme system in sulfur-grown A.ferrooxidans MON-1 cells (2009)
  • Author(s): Sugio T., T.M.Taha, A.Negishi, F.Takeuchi
  • Organizer: 2009年度インターナショナルバイオハイドロメタルルジー ジンポジゥム(IBS2009)
  • Place of Presentation: ベルロッチ(アルゼンチン)
  • Year and Date: 2009-09-17
• [Presentation] Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 (2009)
  • Author(s): Taha, T.M., F.Takeuchi, T.Sugio
  • Organizer: 2009年度インターナショナルバイオハイドロメタルルジージンポジゥム(IBS2009)
  • Place of Presentation: ベルロッチ(アルゼンチン)
  • Year and Date: 2009-09-17
• [Presentation] 鉄酸化細菌Acidithiobacillus ferrooxidans ATCC 23270株におけるtetrathionate hydrolaseが関与する2価鉄の生産 (2009)
  • Author(s): 杉尾剛、ターヘルタハ、竹内文章
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡市(福岡国際会議場)
  • Year and Date: 2009-03-29
• [Presentation] Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from ssulfur-grown Acldithiobacillus ferrooxidans ATCC 23270 (2009)
  • Author(s): Taha, T.M., F.Takeuchi, T.Sugio
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: 福岡市(福岡国際会議場)
  • Year and Date: 2009-03-29
• [Presentation] Ferric Iron Reduction by Tetrathionate is Catalyzed by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells 2nd International Symposium on Microbial Sulfur Metabolism (2009)
  • Author(s): Sugio T., T.M.Taha, F.Takeuchi
  • Place of Presentation: トマール(ポルトガル)
  • Year and Date: 2009-03-17
• [Presentation] Reconstitution of Iron Oxidation System from Sulfur-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells (2008)
  • Author(s): T.M.Taha, F.Takeuchi, T.Sugio
  • Organizer: 平成20年度日本農芸化学会中四国支部大会
  • Place of Presentation: 鳥取市(鳥取大学)
  • Year and Date: 2008-09-13
• [Presentation] A.ferrooxidans D3-2株由来亜硫酸酸化酵素系のc型cytochrome及びaa_3型cytochrome oxidaseからの再構成 (2008)
  • Author(s): 杉尾剛, T.Taha, 早崎琢也, 赤穂亜実, 竹内文章
  • Organizer: 平成20年度日本農芸化学会中四国支部大会
  • Place of Presentation: 鳥取市(鳥取大学)
  • Year and Date: 2008-09-13
• [Presentation] 鉄酸化細菌Acidithiobacillus ferrooxidansにおけるテトラチオン酸ハイドロラーゼの転写解析 (2008)
  • Author(s): 村上知佐、金尾忠芳、上村一雄、杉尾剛
  • Organizer: 平成20年度日本生物工学会大会
  • Place of Presentation: 仙台市(東北学院大学)
  • Year and Date: 2008-08-29
• [Presentation] 鉄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの大腸菌組換え発現と活性型酵素の取得 (2008)
  • Author(s): 金尾忠芳, 松本知恵, 白神久美子, 上村一雄, 杉尾剛
  • Organizer: 平成20年度日本生物工学会大会
  • Place of Presentation: 仙台市(東北学院大学)
  • Year and Date: 2008-08-29