2010 Fiscal Year Final Research Report
Identification and characterization of novel systems for oxygen metabolism and anti-oxidative stress protection in anaerobes
| Project/Area Number |
20580086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
KAWASAKI Shinji 東京農業大学, 応用生物科学部, 准教授 (50339090)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 嫌気性菌 / 抗酸化酵素 / 活性酸素 / 酸素毒性 / Clostridium / Bifidobacterium / 腸内細菌 |
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| Research Abstract |
Our purpose is to know the mechanism of O_2 sensitivity of the obligatory anaerobes Clostridium and Bifidomacterium. Clostridium acetobutylicum, which is well known as an efficient acetone-butanol fermenting bacteria, senses the contamination of O_2 in the growth environments quickly and expresses the O_2 responsive genes. In this study, we investigated the function of O_2-responsive nror operon enzymes by purification of the proteins and characterization of their enzymatic activity. We propose that the obligate anaerobe C. acetobutylicum possesses an efficient multi-enzyme complex that can scavenge O_2 and ROS using NROR as a master electron donor protein. The primary factor responsible for aerobic growth inhibition of Bifidobacterium species is proposed to be the production of H_2O_2 in the growth medium. A H_2O_2-forming NADH oxidase was purified from O_2-sensitive Bifidobacterium bifidum and was identified as a b-type DHOD. The kinetic parameters suggested that the enzyme could be involved in H_2O_2 production in highly aerated environments.
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[Journal Article] Lactobacillus floricola sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Japan2011
Author(s)
Kawasaki, S, K. Kurosawa, M. Miyazaki, C. Yagi, Y. Kitajima, S. Tanaka, T. Irisawa, S. Okada, M. Sakamoto, M. Ohkuma, Y. Niimura
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Journal Title
Int. J. Syst. Evol.Microbiol
Volume: 61
Pages: 1356-1359
Peer Reviewed
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