2010 Fiscal Year Final Research Report

Analysis of biological functions of the 7-methylguanosine modification in 16S rRNA

Research Project

Project/Area Number	20580091
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied microbiology
Research Institution	National Agricultural Research Organization
Principal Investigator	OKSAMOTO Susumu National Agricultural Research Organization, 食品バイオテクノロジー研究領域, ユニット長 (80353986)
Project Period (FY)	2008 – 2010
Keywords	RNA修飾 / 大腸菌 / 放線菌 / 二次代謝
Research Abstract	Bacterial rRNA have many methylated nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. Here, we conducted genetic analyses of this conserved rRNA modification. Apparently, in Escherichia coli, the m7G modification is not essential for its growth under laboratory culture conditions. On the other hand, in Streptomyces coelicolor A3(2), this modification is critical for its viability under high temperature culture conditions. Furthermore, we found that the loss of the m7G modification in 16S rRNA often activates secondary metabolisms in the genus Streptomyces.

(3 results)