2010 Fiscal Year Final Research Report
Purification and analysis of the highly stable hydrogenase as a cytochrome b complex form.
| Project/Area Number |
20580094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
NISHIHARA HIROFUMI Ibaraki University, 農学部, 准教授 (10260465)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ヒドロゲナーゼ / シトクロムb / 蛋白質精製 / 膜蛋白質 / 可溶化 / 水素酸化細菌 / Hydrogenovibrio marinus |
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| Research Abstract |
Hydrogenases have been anticipated for their potential use in hydrogen production and development of alternative hydrogen fuel cell catalyst to noble platinum. In this study, we succeeded in purification of the highly stable hydrogenase as a cytochrome b complex form. It will enable us to perform further crystallogenic and structural study of the enzyme to obtain useful information for hydrogenase stabilization and development of stable mimics.
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