Studies on Reaction Mechanism of Carotenoids and Peroxynitrite In Vivo

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Shizuoka University
• Principal Investigator: ETOH Hideo Shizuoka University, 農学部, 教授 (10076747)
• Co-Investigator(Kenkyū-buntansha): MAOKA Takashi (財)生産開発科学研究所, 主任研究員 (10157159)
• Project Period (FY): 2008 – 2010
• Keywords: 食品化学

Research Abstract

Peroxynitrite, the reaction product of superoxide and nitric oxide, is a powerful oxidant in vivo. Peroxynitrite is known to induce DNA strand scission, protein modification by nitration and hydroxylation and lipid peroxydation in LDL. The in vitro reactivity of lutein, capsanthin and fucoxanthin toward peroxynitrite was investigated, and the reaction products produced by scavenging with peroxynitrite were analyzed. Nitro-carotenoids were isolated from the reaction of these carotenoids with peroxynitrite. These carotenoids inhibited the nitoration of tyrosin by peroxynitrite.

Research Products

• [Journal Article] Nitrocarotenoid and oxazine- carotenoid, reaction products of carotenoids with peroxynitrite (2011)
  • Author(s): Maoka, T., Tsuboi, M., Kulkarni, A., Terada, Y., Kato, K., Nakatsugawa, H., Mori, H., Inakuma, T, Etoh, H.
  • Journal Title: Carotenoid Science Vol.15(印刷中)
  • Peer Reviewed
• [Journal Article] Nitration reaction of lutein with peroxynitrite (2010)
  • Author(s): Tsuboi, M., Etoh, H., Yomoda, Y., Kato, K., Kato, H., Kulkarni, A., Terada, Y., Maoka, T., Mori, H., Inakuma, T.
  • Journal Title: Tetrahedron Lett. Vol.51 Pages: 676-678
  • Peer Reviewed
• [Journal Article] Reaction of astaxanthin with peroxynitrite (2008)
  • Author(s): Hayakawa, T., Kulkarni, A., Terada, Y., Maoka, T., Etoh, H.
  • Journal Title: Biosci.Biotechnol.Biochem. Vol.72 Pages: 2716-2722
  • Peer Reviewed
• [Presentation] Nitrocarotenoid and oxazinecarotenoid, reaction products of carotenoids with peroxynitrite (2010)
  • Author(s): 衛藤英男, 松本岳, 寺田幸正
  • Organizer: 6th International Congress on Pigments in Food, Proceedings of the 6th International Congress
  • Place of Presentation: Hungary, Budapest
  • Year and Date: 20100620-20100624
• [Presentation] カロテノイドによる活性窒素種消去の検討-カロテノイドとペルオキシナイトライトの反応物について- (2010)
  • Author(s): 衛藤英男・眞岡孝至・加藤久喜・中津川広樹・坪井誠・寺田幸正・松本岳・森啓信・細川雅史・宮下和夫
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-30
• [Presentation] アスタキサンチンの自動酸化に関する化学的研究 (2009)
  • Author(s): 衛藤英男・須原三加・中津川広樹・眞岡孝至・寺田幸正・鈴木里英・石倉正治
  • Organizer: 第5回アスタキサンチン研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-18
• [Presentation] ルテインとペルオキシナイトライトの反応について (2009)
  • Author(s): 衛藤英男・四方田雄哉・加藤久喜・眞岡孝至・寺田幸正・森啓信・稲熊隆博
  • Organizer: 第23回カロテノイド研究談話会
  • Place of Presentation: 仙台
  • Year and Date: 2009-09-16
• [Presentation] Reaction of 1,2-epoxylycopene with peroxynitrite (2008)
  • Author(s): Yomota, Y., Kulkarni A., Maoka T., Terada Y., Mori H., Inakuma T., Etoh H
  • Organizer: The15th international symposium on carotenoids, Crotenoid Science
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20080622-20080627
• [Presentation] Reaction of carotenoids with peroxynitrite (2008)
  • Author(s): 衛藤英男
  • Organizer: The15th international symposium on carotenoids (Invited Lecture), Crotenoid Science
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20080622-20080627
• [Book] Nutraceutical Science and Technology10(Nutraceutical Science and Technology10) (2010)
  • Author(s): Maoka, T., Etoh H.
  • Total Pages: 85-97
  • Publisher: CRC Press, Canada
• [Book] カロテノイドの科学と最新応用技術(第2編カロテノイドの機能、第4章カロテノイドによるペルオキシナイトライト消去の化学的機構) (2009)
  • Author(s): 衛藤英男
  • Total Pages: 132-137
  • Publisher: シーエムシー出版