Elucidation of a novel anti-allergic mechanism of pulverized dietary fiber and its application

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Hiroshima University
• Principal Investigator: KAWAMOTO Seiji Hiroshima University, 大学院・先端物質科学研究科, 准教授 (90294537)
• Project Period (FY): 2008 – 2010
• Keywords: 微粉砕食物繊維 / グルコマンナン / アトピー性皮膚炎 / 機能性食品

Research Abstract

In the present study, I analyzed the mode of anti-allergic mechanism of pulverized dietary fiber, especially focused on konjac glucomannan (KGM) as a model diet. I found that dietary pulverized KGM (PKGM) had beneficial effect on the prevention of atopy-associated autoimmune response as well as on the suppression of nasal mast cell infiltration in the allergic rhinitis. I also found that PKGM supplementation induced T cell tolerance against excessive antigen-specific proliferative response. These results suggest that PKGM acts on multiple immune targets to fulfill its anti-inflammatory potency. I also determined optimal particle size of PKGM to achieve its anti-allergic effect, and generated a trial product of PKGM-supplemented food.

Research Products

• [Journal Article] Sake lees fermented with lactic acid bacteria prevents allergic rhinitis-like symptoms and IgE-mediated basophil degranulation. (2011)
  • Author(s): Kawamoto S., Kaneoke M., Ohkouchi K., Amano Y., Takaoka Y., Kume K., Aki T., Watanabe K., Kadowaki M., Hirata D., Ono K.
  • Journal Title: Biosci. Biotechnol. Biochem. 75 Pages: 140-144
  • Peer Reviewed
• [Journal Article] Molecular cloning and immunochemical characterization of a new Japanese cedar pollen allergen homologous to plant subtilisin-like serine protease. (2010)
  • Author(s): Ibrahim A.R.N., Kawamoto S., Mizuno K., Shimada Y., Rikimaru S., Onishi N., Hashimoto K., Aki T., Hayashi T., Ono K.
  • Journal Title: World Allergy Organization J. 3 Pages: 262-265
  • Peer Reviewed
• [Journal Article] Immunological aspects and therapeutic significance of an auto-Ab against histone H1 in a rat model of Con A-induced hepatitis. (2010)
  • Author(s): Nakano T., Goto S., Lai C.Y., Hsu L.W., Takaoka Y., Kawamoto S., Chiang K.C., Shimada Y., Ohmori N., Goto T., Sato S., Ono K., Cheng Y.F., Chen C.L.
  • Journal Title: Immunology 129 Pages: 547-555
  • Peer Reviewed
• [Journal Article] Molecular cloning and immunochemical characterization of a novel major Japanese cedar pollen allergen belonging to the aspartic protease family. (2010)
  • Author(s): Ibrahim A.R.N., Kawamoto S., Aki T., Shimada Y., Rikimaru S., Onishi N., Babiker E.E., Oiso I., Hashimoto K., Hayashi T., Ono K.
  • Journal Title: Int.Arch.Allergy Immunol. 152 Pages: 207-218
  • Peer Reviewed
• [Journal Article] A new lipid transfer protein homolog identified as an IgE-binding antigen from Japanese cedar pollen. (2010)
  • Author(s): Ibrahim A.R.N., Kawamoto S., Nishimura M., Pak. S., Aki T., Diaz-Perales A., Salcedo G., Asturias J.A., Hayashi T., Ono K.
  • Journal Title: Biosci. Biotechnol. Biochem. 74 Pages: 504-509
  • Peer Reviewed
• [Journal Article] Development of autoantibody responses in NC/Nga mice : its prevention by pulverized konjac glucomannan feeding. (2008)
  • Author(s): Onishi N., Kawamoto S., Suzuki H., Hide M., Ono K.
  • Journal Title: Arch.Dermatol. Res. 300 Pages: 95-99
  • Peer Reviewed
• [Journal Article] アレルギーワクチンの開発に向けて (2008)
  • Author(s): 河本正次、秋庸裕、小埜和久
  • Journal Title: BIO INDUSTRY 25 Pages: 69-77
• [Presentation] Recombinant allergens for immunotherapy. (2010)
  • Author(s): Kawamoto S., Aki T., Ono K.
  • Organizer: BIT's 1st Annual World Congress of Immunodiseases and Therapy (invited lecture)
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-05-15
• [Presentation] Recombinant allergens for future component-resolved molecular diagnosis and tailor-made immunotherapy. (2008)
  • Author(s): Kawamoto S., Aki T., Ono K.
  • Organizer: BIT's 1st Annual World Congress of Vaccine 2008 (invited lecture)
  • Place of Presentation: Foshan, Guandong, China
  • Year and Date: 2008-12-04