2010 Fiscal Year Final Research Report
Improvement of the saprophytic ability in Matsutake mushroom by cross breeding technology
| Project/Area Number |
20580175
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tottori University |
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Principal Investigator |
AIMI Tadanori Tottori University, 農学部, 教授 (90264928)
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| Project Period (FY) |
2008 – 2010
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| Keywords | マツタケ / プロトプラスト / グルコアミラーゼ遺伝子 / 育種 / 一核株 / 核相 / 再生 / ホメオドメインタンパク質 |
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| Research Abstract |
Several genes related for mating type and starch metabolism were identified to use for investigation of mating type and karyotype in the T.matsutake isolates. From these results indicated that the T.matsutake isolates from the fruiting body tissue were essentially heterokaryon. Secondly, we examined the condition of protoplast formation and its regeneration in order to isolate homokaryon in the ectomycorrhizal fungus Tricholoma matsutake.
As the results, when trehalose was used as osmotic stabilizer, the protoplast formation and mycelial growth of T.matsutake was promoted, however, xylose completely inhibited mycelial growth.
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