2010 Fiscal Year Final Research Report
A study on the mechanisms of growth in super salmon and its application for improvement of muscle production
| Project/Area Number |
20580219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
NAKANO Toshiki Tohoku University, 大学院・農学研究科, 助教 (10217797)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAKAWA Hitoshi 東北大学, 大学院・農学研究科, 准教授 (40206280)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 農林水産物 / 水産学 / 栄養学 / 組換え食品 / バイオテクノロジー / 生理学 |
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| Research Abstract |
Growth in fish is regulated by the growth hormone (GH)/growth hormone receptor (GHR)/insulin-like growth factor (IGF)-I axis. This GH-IGF-I axis is influenced by several factors. With regard to hormonal effect, it is known that salmon transgenic for GH show increased growth. However, very little is known about the expression patterns of growth-related genes in GH transgenic fish. The present study examined the daily expression patterns of mRNAs for the growth-related genes after feeding in GH transgenic coho salmon (Oncorhynchus kisutch). GH mRNA was detected in both liver and muscle as well as in the pituitary from transgenic fish. GHR mRNA levels in the liver and muscle were higher in transgenic fish than in non-transgenic wild type fish but lower in the pituitary. The expression level of hepatic IGF-I mRNA was greater in transgenic fish. The daily GH, GHR and IGF-I mRNA expression patterns reached their peak at roughly 4-8 h after feeding, however, GHR and IGF-I mRNA expressions in the liver was observed to be irregular in non-transgenic fish. These results suggest that the daily expression patterns for growth-related genes are particularly pronounced in GH transgenic fish. Furthermore, our findings would be useful for consideration of the time of feeding and the optimum time of day for tissue sampling for analysis of growth-related genes in fish.
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Research Products
(15 results)
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[Journal Article] Effect of stress on growth related gene expressions in coho salmon2008
Author(s)
Nakano T, Kameda, M., Yamaguchi, T., Sato, M., Afonso, L., Iwama, G.K., Devlin, R.H.
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Journal Title
In Proceedings of 5th World Fisheries Congress, Yokohama (CD-ROM)
Pages: 3f-1-2
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[Presentation] Daily expression patterns of growth-related genes in transgenic coho salmon2010
Author(s)
Nakano T., Shoji, Y., Shirakawa H., Suda Y., Yamaguchi T., Sato M., Devlin, R.H.
Organizer
International Conference of French-Japanese Society for Oceanography (Techno-Ocean 2010), Abstracts
Place of Presentation
Kobe International Convention Center, Kobe, Japan(CD-ROM)
Year and Date
20101014-20101016
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[Presentation] Effect of stress on growth related gene expressions in coho salmon2008
Author(s)
Nakano T., Kameda M., Yamaguchi T., Sato M., Afonso, L., Iwama, G.K., Devlin, R.H.
Organizer
5th World Fisheries Congress, Abstracts p.196
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Year and Date
20081020-20081024
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[Presentation] Effects of heat shock on growth hormone receptor expression in coho salmon2008
Author(s)
Kameda M., Nakano T., Yamaguchi T., Sato M., Afonso, L., Iwama, G.K., Devlin, R.H.
Organizer
5th World Fisheries Congress, Abstracts p.389
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Year and Date
20081020-20081024
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