A study on fish lipocalin protein which bind to low molecular lipophilic compound

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580221
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kyushu University
• Principal Investigator: YUJI Oshima Kyushu University, 農学研究院, 教授 (70176874)
• Project Period (FY): 2007 – 2010
• Keywords: 静菌 / 生体防御 / 低分子結合性 / リポカリン

Research Abstract

Tributyltin (TBT) is well-known as a strong endocrine disruptor and has been detected at high concentrations in the blood of fishes living in coastal areas. In our previous studies, we identified TBT-binding proteins (TBT-bp1 and 2) bound to TBT in the blood of Japanese flounder (Paralichthys olivaceus) and determined that TBT-bps were have a function that would bind to TBT and then be excreted into the mucus. TBT-bps are a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1and -bp2 (rTBT-bp1, 2) in a baculovirus expression system and purified the protein from the hemolymph of silkworm larvae injected with recombinant baculovirus. From the results of binding assay of rTBT-bp 2 to some chemicals, TBT-bp2 show affinity to TBT but not to lauric acid, palmitic acid and tearic acid. Furthermore, rTBT-bp1 may inhibit the growth of fish dieseas bacteria Edwardsiella tarda, suggesting bacteriostasis activity.

Research Products

• [Journal Article] Purification and characterization of tributyltin-binding protein of tiger puffer (2011)
  • Author(s): Oba Y, Yamauchia A, Hashiguchi Y, Satonea H, Miki S, Nassef M, Shimasaki Y, Kitano T, Nakao M, Kawabata S, Honjo T, Oshima Y,.
  • Journal Title: Takifugu rubripes. Comp Biol phys C. 153 Pages: 17-23
• [Journal Article] Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. (2011)
  • Author(s): Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y.
  • Journal Title: Aquat Toxicol 103 Pages: 79-84
• [Journal Article] Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes. (2011)
  • Author(s): Nassef M, Kato-Unoki Y, Furuta T, Nakayama K, Satone H, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Zoological Science 28 Pages: 281-285
• [Journal Article] Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder (2008)
  • Author(s): Satone H, Oshima Y, Shimasaki Y, Tawaratsumida T, Oba Y, Takahashi E, Kitano T, Kawabata S, Kakuta Y, Honjo T.
  • Journal Title: Paralichthys olivaceus. Aquat Toxicol 90 Pages: 292-299
• [Journal Article] Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27.
  • Author(s): Nassef M, Tawaratsumida T, Oba Y, Satoe H, Nakayama K, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Mar Poll Bull 62 Pages: 412-414
• [Presentation] 魚類リポカリンは異物(トリブチルスズやフグ毒)と結合し体表粘液から排泄する機能を持つ (2010)
  • Author(s): 大嶋雄治
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-16
• [Presentation] The detoxicification and excreation function of tributyltin binding protein in Japneseflounder Paralichthyes olivaceus. (2010)
  • Author(s): Oshima Y, Satone H, Yumi Oba Y, Lee JM, Kusakabe T, Kawabata S, Shimasaki Y, Suzuki N, Sasayama Y.
  • Organizer: Society of Environmental Toxicology and Chemistry Asia Pacific 2010
  • Place of Presentation: 広州(中華人民共和国)
  • Year and Date: 2010-06-04
• [Presentation] Induction of the tributyltin-binding protein 2 of Japanese flounder, Paralichthys olivaceus by exposure of tributyltin-d27. (2010)
  • Author(s): Y Oshima, T.Tawaratsumida, Y Oba, H Satone, Y Shimasaki
  • Organizer: 6^<th> International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: 香港(中華人民共和国)
  • Year and Date: 2010-05-31
• [Presentation] Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. (2010)
  • Author(s): Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y
  • Organizer: Society of Environmental Toxicology and Chemistry.
  • Place of Presentation: Seville Spain
  • Year and Date: 2010-05-22
• [Presentation] Analysis of the tribu-tyltin-binding protein gene of Japanese flounder, Paralichthys olivaceus. (2010)
  • Author(s): Oshima, Y., Ooba Y., Satone H., Shimasaki, Y., Hashiguchi, Y.
  • Organizer: Society of Environmental Toxicology and Chemistry.
  • Place of Presentation: Seville Spain
  • Year and Date: 2010-05-22
• [Presentation] Expression of tributyltin-binding protein type 1 in silkworms using by a baculovirus gene expression system. (2009)
  • Author(s): Hina Satone, Jae Man Lee, Takahiro Kusakabe, Yumi Oba, Yohei Shimasaki, Yuji Oshima
  • Organizer: 14th International Symposium on Toxicity Assessment.
  • Place of Presentation: Mettz, France
  • Year and Date: 2009-08-30