Study on a novel lipid signaling pathway and that possible regulatory mechanism in chicken hepatocytes-Basal knowledge for production of functional meats-

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580302
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SATO Kan Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (20250730)
• Project Period (FY): 2008 – 2010
• Keywords: 代謝 / 内分泌制御

Research Abstract

Apolipoprotein B (apoB) is an integral protein component of very low density lipoprotein (VLDL), a key regulator of fat mass in farm animals. ApoB mRNA expression and apoB protein synthesis in hepatocytes are relatively constitutive and generally does not change under conditions that alter apoB secretion. Therefore, VLDL assembly is regulated largely at co- and post-translational levels. In present study, we investigated the identification and characterization of novel lipid signaling pathway to impair the apoB mRNA synthesis. ApoB mRNA expression in chickens supplemented with octanoate (C8:0)- and decanoate (C10:0)-triacylglycerols in diets significantly decreased compared to that in control group. This result suggested that the impairment of apoB mRNA expression by medium chain fatty acids (MCFAs) in chicken causes in normal and physiological condition in chickens. The two MCFAs responsible elements were determined in chicken apoB promoter, which was cited in -200 and -1000～-2000bp from coding region. These results clearly show a novel lipid signaling pathway, which is associated by MCFAs, and the signaling impaired apoB mRNA expression in chicken hepatocytes.

Research Products

• [Journal Article] Regulation of bile acid, cholesterol, and fatty acid synthesis in chicken primary hepatocytes by different concentrations of T0901317, an agonist of liver X receptors. (2011)
  • Author(s): Sato K, Kamada T.
  • Journal Title: Comp Biochem Physiol A. 158. Pages: 201-206.
  • Peer Reviewed
• [Journal Article] Tissue distribution of lipase genes related to triglyceride metabolism in laying hens (Gallus gallus). (2010)
  • Author(s): Sato K, Seol HS, Kamada T.
  • Journal Title: Comp Biochem Physiol B. 155. Pages: 62-66.
  • Peer Reviewed
• [Journal Article] Characterization of chicken portomicron remnant and very low density lipoprotein remnant. (2009)
  • Author(s): Sato K., Suzuki K., Akiba Y.
  • Journal Title: Journal of Poultry Science. 46. Pages: 35-39
  • Peer Reviewed
• [Journal Article] Role of peroxisome proliferator-activated receptor beta/delta in chicken adipogenesis. (2009)
  • Author(s): Sato K, Yonemura T, Ishii H, Toyomizu M, et al.
  • Journal Title: Comp Biochem Physiol A. 154. Pages: 370-375.
  • Peer Reviewed
• [Journal Article] Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets. (2009)
  • Author(s): Sato K, Takahashi K, Tohno M, Miura Y, Kamada T, Ikegami S, Kitazawa H.
  • Journal Title: Poultry Science. 88. Pages: 2532-2538.
  • Peer Reviewed
• [Journal Article] Adipose tissue fat accumulation is reduced by a single intraperitoneal injection of PPARgamma agonist when given to newly hatched chickens. (2008)
  • Author(s): Sato, K., Matsushita, K., Matsubara, Y., Kamada, T., Akiba, Y.
  • Journal Title: Poultry Science. 87. Pages: 2281-2286.
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression of angiopoietin-like protein 3 in the chicken, Gallus gallus. (2008)
  • Author(s): Sato, K., Seol, HS., Sato, K., Kamada, T., Akiba, Y.
  • Journal Title: Gen.Comp.Endocrinology. 158. Pages: 102-107.
  • Peer Reviewed
• [Remarks] ホームページ等