2011 Fiscal Year Final Research Report
Establishment of the technique for sperm cryopreservation in Okinawan native Agu pig
| Project/Area Number |
20580312
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | University of the Ryukyus |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2008 – 2011
|
| Keywords | 精子 / アグー / ブタ / 凍結保存 / 細胞障害 / 酸化ストレス / アポトーシス |
|---|
| Research Abstract |
The technical establishment of boar sperm cryopreservation isindispensable for effective breeding of the scarce Okinawan native Agu pig. In this study, the addition of ascorbic acid 2-Ο-α-glucoside(AA-2G) and low-density lipoprotein(LDL) tothe freezing extender reduced the plasma membrane damage in post-thaw Agu sperm as aconsequence of the protection of sperm against extracellular oxidative stress and cold shockdamage, respectively, during cryopreservation. Moreover, treatment with an artificial anti-cell death protein(PTD-FNK protein) prevented the mitochondrial dysfunction leading to the apoptotic-cell death during cryopreservation. Therefore, these results indicate that the post-thaw qualities of fragile Agu sperm is effectively improved throughthe established techniques in this study.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Remarks] いのちドラマチック第1集,福岡伸一監修, NHK「いのちドラマチック」制作班編,「アグー豚」, p103-112,木楽舎, 2011年
