2010 Fiscal Year Final Research Report
Mechanisms of sexual dimorphism of IFNτ secretion in bovine blastocyst
| Project/Area Number |
20580333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
KIMURA Koji National Agricultural Research Organization, 家畜育種増殖研究チーム, 主任研究員 (50355070)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ウシ / 胚 / インターフェロン / 性 / 転写因子 |
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| Research Abstract |
Sexual dimorphic expression of IFNτ of bovine embryo was not caused via the sexual difference of expression of transcription factors, which affect IFNτ gene expression. However, the expression of Dnmt3a, one of DNA methyltransferases, of bovine male blastocysts was significantly higher than that of female embryos. Moreover, addition of 5-azadeoxycytidene, an inhibitor of DNA methyltransferase, to the culture medium of bovine embryo abolished the sexual dimorphic secretion of IFNτ from bovine embryos. These results suggest that the DNA methylation of bovine embryo genome might be involved in the occurrence of sexual dimorphic expression of IFNτ in bovine embryos.
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