Genome-wide screening for the virulence markers of Streptococcus suis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: SEKIZAKI Tsutomu The University of Tokyo, 大学院・農学生命科学研究科, 教授 (70355163)
• Co-Investigator(Renkei-kenkyūsha): OSAKI Makoto 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所, 主任研究員 (80355164) ; TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所, 主任研究員 (60414728)
• Project Period (FY): 2008 – 2010
• Keywords: 獣医細菌学 / 分子遺伝学 / レンサ球菌

Research Abstract

Genomic DNAs of two S. suis virulent strains belonging two genetically different clones were subtracted by a genomic DNA of an avirulent strain. Twenty-three genes selected were found to exist in only virulent strains. These genes were knocked out via single-cross over, and one of the mutants showed remarkable loss of virulence. However, virulence of the knock out mutant via double-cross over was the same as its parent stain.

Research Products

• [Journal Article] The minor pilin subunit Sgp2 is necessary for assembly of the pilus encoded by the srtG cluster of Streptococcus suis (2011)
  • Author(s): Okura M., Osaki M., Fittipaldi N., Gottschalk M., Sekizaki T., Takamatsu D.
  • Journal Title: J.Bacteriol. 193 Pages: 822-831
  • Peer Reviewed
• [Journal Article] Mutations in the gene encoding the ancillary pilin subunit of the Streptococcus suis srtF cluster result in pili formed by the major pilin subunit only (2010)
  • Author(s): Fittipaldi N., Takamatsu D., Doinguez-Punaro M., Lecours M., Montpetit D., Osaki M., Sekizaki T., Gottschalk M.
  • Journal Title: PLoS ONE 5 Pages: e8426
  • Peer Reviewed
• [Journal Article] 動物に潜む病原レンサ球菌Streptococcus suisについて (2010)
  • Author(s): 関崎勉
  • Journal Title: BMSA会誌 2(1) Pages: 7-12
• [Journal Article] 豚レンサ球菌の病原因子と強毒株マーカーについて (2010)
  • Author(s): 関崎勉
  • Journal Title: 日生研たより 56(1) Pages: 5-12
• [Journal Article] Genetic organization and preferential distribution of putative pilus gene clusters in Streptococcus suis (2009)
  • Author(s): Takamatsu D., Nishino H., Ishiji T., Osaki M., Fittipaldi N., Gottschalk M., Tharavichitkul P., Takai S., Sekizaki T.
  • Journal Title: Vet.Microbiol. 138 Pages: 132-139
  • Peer Reviewed
• [Journal Article] 動物に潜在する病原レンサ球菌について (2009)
  • Author(s): 関崎勉
  • Journal Title: 宮城県獣医師会会報 62(4) Pages: 213-217
• [Journal Article] 豚レンサ球菌の多様性と病原性に関する解析 (2009)
  • Author(s): 関崎勉
  • Journal Title: 化学療法の領域 25(11) Pages: 119-126
• [Journal Article] Significant contribution of the pgdA gene to the virulence of 2 Streptococcus suis (2008)
  • Author(s): Fittipaldi N., Sekizaki T., Takamatsu D., Dominguez-Punaro M. C., Harel J., Bui N. K., Vollmer W., Gottschalk M.
  • Journal Title: Mol.Microbiol. 70 Pages: 1120-1135
  • Peer Reviewed
• [Journal Article] D-alanylation of the lipoteichoic acid contributes to the virulence of Streptococcus suis (2008)
  • Author(s): Fittipaldi N., Sekizaki T., Takamatsu D., Harel J., Dominguez-Punaro M. C., Von Aulock S., Draing C., Marois C., Kobisch M., Gottschalk M.
  • Journal Title: Infect.Immun. 76 Pages: 3587-3594
  • Peer Reviewed
• [Journal Article] Allelic variation and the prevalence of serum opacity factor among Streptococcus suis population (2008)
  • Author(s): Takamatsu D., Osaki M., Tharavichitkul P., Takai S., Sekizaki T.
  • Journal Title: J.Med. Microbiol. 57 Pages: 488-494
  • Peer Reviewed
• [Journal Article] Disruption of srtA gene in Streptococcus suis results in decreased interactions with endothelial cells and extracellular matrix proteins (2008)
  • Author(s): Vanier G., Sekizaki T., Dominguez-Punaro M., Esgleas M., Osaki M., Takamatsu D., Segura M., Gottschalk M.
  • Journal Title: Vet.Microbiol. 127 Pages: 417-424
  • Peer Reviewed
• [Journal Article] Streptococcus suis in humans, Thailand (2008)
  • Author(s): Takamatsu D., Wongsawan K., Osaki M., Nishino H., Ishiji T., Tharavichitkul P., Khantawa B., Fongcom A., Takai S., Sekizaki T.
  • Journal Title: Emerg.Infect.Dis. 14 Pages: 181-183
  • Peer Reviewed
• [Presentation] Requirement of the Minor Pilin Subunit Sgp2 for Assembly of the Pilus Encoded by the srtG Cluster of Streptococcus suis (2010)
  • Author(s): Okura M., Osaki M., Sekizaki T.,, Takamatsu D.
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center, Hyogo, Japan
  • Year and Date: 20100907-20100910
• [Presentation] Streptococcus infection (2010)
  • Author(s): 関崎勉
  • Organizer: Workshop, Infectious Diseases in Animal and Food Safety in East Asia, 9th International Veterinary Immunology Symposium
  • Place of Presentation: Tower Hall Funabori, Tokyo
  • Year and Date: 20100816-20100820
• [Presentation] Threat of Streptococcus suis infection (2010)
  • Author(s): 関崎勉
  • Organizer: International Conference on Food Security During Challenging Times
  • Place of Presentation: Universiti Putra Malaysia, Kuala Lumpur, Malaysia
  • Year and Date: 20100705-20100707
• [Presentation] 線毛関連遺伝子のプロファイリングによる豚レンサ球菌強毒株の識別 (2010)
  • Author(s): 高松大輔,大西英高,大倉正稔,大崎慎人,関崎勉
  • Organizer: 第19回Lancefieldレンサ球菌研究会
  • Place of Presentation: 国立国際医療センター研究所,東京
  • Year and Date: 20100625-20100626
• [Presentation] srtG遺伝子群によるStreptococcus suisの線毛形成及び線毛組立てに必要なマイナーサブユニットSgp2についての解析 (2010)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高松大輔
  • Organizer: 第19回Lancefieldレンサ球菌研究会
  • Place of Presentation: 国立国際医療センター研究所,東京
  • Year and Date: 20100625-20100626
• [Presentation] Streptococcus suisのsrtG遺伝子群の線毛形成に関する解析 (2010)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高松大輔
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(東京大学医科研)
  • Year and Date: 20100327-20100329
• [Presentation] Significant Contribution of Minor Pilin Subunit Sgp2 to Efficient Assembly of the Pilus Encoded by srtG Cluster of Streptococcus suis (2010)
  • Author(s): Okura M., Osaki M., Fittipaldi N., Gottschalk M., Sekizaki T.,, Takamatsu D.
  • Organizer: US-Japan Co-Operation of Natural Resources, Conference on Animal Hygiene and Infectious Diseases
  • Place of Presentation: National Animal Disease Center, Ames, Iowa, USA
  • Year and Date: 2010-10-05
• [Presentation] Streptococcus suisのsrtG遺伝子群による線毛様構造の発現及びマイナー線毛サブユニットSgp2の線毛形成に重要な領域の特定 (2009)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高松大輔
  • Organizer: 第92回日本細菌学会関東支部総会
  • Place of Presentation: 東京医科歯科大学(東京医科歯科大学医学部)
  • Year and Date: 20091105-20091106
• [Presentation] Streptococcus suisの線毛関連遺伝子群の分布及びsrtG遺伝子群の線毛形成に関する解析 (2009)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高井伸二,高松大輔
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋国際会議場(名古屋大学医学部)
  • Year and Date: 20090312-20090314
• [Presentation] Streptococcus suisのofs遺伝子の多様性と人および豚由来株内での遺伝子の分布状況 (2008)
  • Author(s): 高松大輔,大崎慎人,高井伸二,関崎勉
  • Organizer: 第91回日本細菌学会関東支部総会
  • Place of Presentation: 千葉県「生命の森リゾート」(千葉大学薬学部)
  • Year and Date: 20081023-20081024
• [Presentation] Streptococcus suisの線毛関連遺伝子群の分布およびsrtG遺伝子群の線毛形成に関する解析 (2008)
  • Author(s): 大倉正稔,大崎慎人,関崎勉,高井伸二,高松大輔
  • Organizer: 第91回日本細菌学会関東支部総会
  • Place of Presentation: 千葉県「生命の森リゾート」(千葉大学薬学部)
  • Year and Date: 20081023-20081024
• [Presentation] Zoonosisとしての豚レンサ球菌症 (2008)
  • Author(s): 関崎勉
  • Organizer: 日本細菌学会関東支部会2008年第1回シンポジウム
  • Place of Presentation: 国立国際医療センター研究所
  • Year and Date: 2008-10-17
• [Book] 世界の食料・日本の食料(日本農学会編)(第9章 動物感染症の制御と畜産物の安全)(リーズ21世紀の農学) (2010)
  • Author(s): 関崎勉
  • Total Pages: 151-166
  • Publisher: 養賢堂
• [Book] 食の安全科学の展開-食のリスク予測と制御に向けて-(『東京大学 食の安全研究センター』編)(第2章 動物由来レンサ球菌の脅威) (2010)
  • Author(s): 関崎勉
  • Total Pages: 20-27
  • Publisher: シーエムシー出版
• [Remarks] ホームページ等