2010 Fiscal Year Final Research Report
The development of a ultra-sensitive diagnostic technique for BSE(Mad cow disease)
| Project/Area Number |
20580354
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YAMAGUCHI Yoshitaka The University of Tokushima, 疾患酵素学研究センター, 助教 (90432765)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAGUCHI Suehiro 徳島大学, 疾患酵素学研究センター, 教授 (60274635)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 獣医学 / 蛋白質 / 脳神経疾患 |
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| Research Abstract |
This research was designed to develop a ultra-sensitive detection method for prion disease. We tried to establish the detection method with high sensitivity and singularity, according to amplify the DNA which uniquely bound to the prion protein and decide the sequence. Though the DNA sequence GGATG was identified from REPSA method as a candidate of consensus sequence, it didn't support sufficient for reproducibility and specificity in the latest study.
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Research Products
(7 results)
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[Journal Article] Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions.2010
Author(s)
Koji Fujita, Yoshitaka Yamaguchi, suyoshi Mori, Naomi Muramatsu, Takahito Miyamoto, Masashi Yano, Hironori Miyata, Akira Ootsuyama, Makoto Sawada, Haruo Matsuda, Ryuji Kaji , Suehiro Sakaguchi.
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Journal Title
Cellular and Molecular Neurobiology in press
Peer Reviewed
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[Journal Article] Dominant-negative effects of the N-terminal half of prion protein on neurotoxicity of prion protein-like rotein/doppel in mice.2008
Author(s)
Yoshikawa D, Yamaguchi N, Ishibashi D, Yamanaka H, Okimura N, Yamaguchi Y, Mori T, Miyata H, Shigematsu K, Katamine S, Sakaguchi S.
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Journal Title
J Biol Chem. 283(35)
Pages: 24202-11
Peer Reviewed
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