2010 Fiscal Year Final Research Report
Saccharification of biomass with enzymes fused to carbohydrate-binding modules
| Project/Area Number |
20580362
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Mie University |
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Principal Investigator |
KARITA Shuichi Mie University, 大学院・地域イノベーション学研究科, 教授 (90233999)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 糖質結合モジュール / 等温滴定熱量計 / セルラーゼ / セルロース結合モジュール / バイオマス糖化 |
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| Research Abstract |
Cellulose-binding modules, family 3,17,28, and 44, were characterized with the macro-array method, isothermal titration calorimetry;and Langmuir isotherman alysis. To determine binding-sites on plant cell wall, these cellulose-binding modules were fused to a fluorescent protein. Fluorescent fused cellulose binding modules bound to different parts of plant cell wall and discriminated cell types of plant tissues. These results indicated that cellulose-binding modules can recognize a diffbrent structure on plant cell walls, whereas these modules have similar binding specificities. A catalytic domain belonging to glycoside hydrolase family 5 was fused to cellulose binding modules. These artificial enzymes showed their activities were influenced by fused modules.
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[Journal Article] Family 17 and 28 carbohydrate-binding modules discriminate different cell wall sites in sweet potato roots.2010
Author(s)
Araki, Y., Karita S., Tsuchiya, T., Kondo, M., Goto, M.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 74
Pages: 802-805
Peer Reviewed
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[Journal Article] Characterization of family 17 and family 28 carbohydrate-binding modules from Clostridium josui Cel5A.2009
Author(s)
Araki, Y., Karita S., Tanaka, A., Kondo, M., Goto, M.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 73
Pages: 1028-1032
Peer Reviewed
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