2010 Fiscal Year Final Research Report
Analysis of denitrification activity expression factor of denitrification hotspot at groundwater environment based on molecular ecology
| Project/Area Number |
20580368
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | National Institute for Agro-Environmental Sciences |
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Principal Investigator |
NAKAJIMA Yasuhiro National Institute for Agro-Environmental Sciences, 物質循環研究領域, 主任研究員 (10354086)
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| Co-Investigator(Kenkyū-buntansha) |
EGUCHI Sadao 独立行政法人農業環境技術研究所, 物質循環研究領域, 主任研究員 (30354020)
HOSHINO Yuko (高田 裕子) 独立行政法人農業環境技術研究所, 生物生態機能研究領域, 研究員 (40354104)
MORIMOTO Sho 独立行政法人農業環境技術研究所, 生物生態機能研究領域, 研究員 (00354118)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 硝酸態窒素 / 脱窒 |
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| Research Abstract |
We developed a method of evaluating denitrification activity by analysis of nitrogen and oxygen stable isotope ratio of nitrate to search denitrification hotspot which has nitrate reduction activity.
We searched out denitrification hotspot from alluvium-diluvium interface and took subsoils. DNA was extracted from subsoil and PCR-DGGE profiles were made for analysis of denitrification hotspot-specific bacterial ecosystems. Subsoils near denitrification hotspot were incubated with nitrate-rich groundwater, results showed that nitrate was reduced within two weeks.
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