Construction of chromosome engineering for Aspergillus oryzae with its telomere function

2010 Fiscal Year Final Research Report

• Project/Area Number: 20580374
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: National Agricultural Research Organization
• Principal Investigator: KUSUMOTO Ken-ichi National Agricultural Research Organization, 応用微生物研究領域, ユニット長 (80353978)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Satoshi 独立行政法人農業・食品産業技術総合研究機構・食品総合研究所, 応用微生物研究領域・糸状菌ユニット, 主任研究員 (90353979)
• Project Period (FY): 2008 – 2010
• Keywords: アスペルギルス / オリゼ / テロメア / 染色体工学

Research Abstract

Aspergillus oryzae is known as an important microorganism utilized as koji mold in fermentation food industry. Person in charge previously found that the terminal sequences known as telomere is a novel repeat sequence with twelve nucleotides as a repeat unit. The telomeric sequence of Aspergillus oryzae, whose length was four times of the native telomere, was synthesized. It was linked to the linear vector. Then the transformants by the vector revealed to have chromosomes whose specific terminal region was successfully deleted.