2010 Fiscal Year Final Research Report

Development of Universal Type of High Speed Countercurrent Chromatograph and Its Application to Separation of Polar Compounds

Research Project

Project/Area Number	20590040
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Physical pharmacy
Research Institution	Nihon University
Principal Investigator	SHINOMIYA Kazufusa Nihon University, 薬学部, 准教授 (70215995)
Project Period (FY)	2009 – 2010
Keywords	分析化学
Research Abstract	Universal type of high-speed countercurrent chromatograph (universal HSCCC) was newly designed and fabricated in our university, and applied to protein separation using an aqueous-aqueous polymer phase system. The present apparatus can produce a synchronous planetary motion of coiled separation column by selecting the normal forward rotation (P_<forward>) or the reversed backward rotation (P_<backward>) against the revolution direction. No stationary phase retention was obtained in P_<backward> rotation, while sufficient protein separation was achieved in P_<forward> rotation. In this P_<forward> rotation, partition efficiency obtained by the eccentric coil assembly was better than that obtained by the toroidal coil assembly. By improving the apparatus to the nonsynchronous HSCCC, which can produce different rotation and revolution speeds independently, increased partition efficiency in P_<forward> rotation was obtained more than the original universal HSCCC. The nonsynchronous HSCCC was successfully fabricated by introducing the new rotary seal system in the distribution line of flow tube.