Roles of Collgenase-3/MMP13 for Collagenolysis in On-set Osteoporosis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: INADA Masaki Tokyo University of Agriculture and Technology, 大学院・工学研究院, 准教授 (80401454)
• Co-Investigator(Renkei-kenkyūsha): MIYAURA Chisato 東京農工大学, 大学院・工学研究院, 教授 (20138382)
• Project Period (FY): 2008 – 2010
• Keywords: 骨粗鬆症 / 病態生理学 / マトリックス / コラーゲン

Research Abstract

This proposal investigated roles of bone cell expressed collagenase 3 on bone remodeling and its related diseases using MMP13 gene deficient mice (MMP13KO). In the mouse models of osteoporosis, the result indicates suppressive phenotypes of bone resorption in MMP13KO.

Research Products

• [Journal Article] Nobiletin, a polymethoxy flavonoid, suppresses bone resorption by inhibiting NFκB-dependent prostaglandin E synthesis in osteoblasts and prevents bone loss due to estrogen deficiency. (2011)
  • Author(s): Harada S, Tominari T, Matsumoto C, Hirata M, Takita M, Inada M, Miyaura C
  • Journal Title: J.Pharmacol.Sci. 115(1) Pages: 89-93
  • Peer Reviewed
• [Journal Article] Cell Shape and Matrix Production of Fibroblasts cultured on fibroin-organized scaffold with type-II-turn structured(Ala-Gly-Ala -Gly-Ser-Gly)n sequences. (2010)
  • Author(s): Hirata M, Kobayashi M, Matsumoto C, Miyaura C, Asakura T, Inada M
  • Journal Title: J.Health Sci. 56(6) Pages: 1-7
  • Peer Reviewed
• [Journal Article] Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins. (2010)
  • Author(s): Kim J, Kobayashi M, Fukuda M, Ogasawara D, Kobayashi N, Han S, Nakamura C, Inada M, Miyaura C, Ikebukuro K, Sode K
  • Journal Title: Prion. 4(1) Pages: 26-31
  • Peer Reviewed
• [Journal Article] Role of prostaglandin E in Receptor Activator of Nuclear Factor κB Ligand (RANKL) expression in osteoblasts induced by cell adhesion to bone marrow B-lymphocytes. (2009)
  • Author(s): Hirata M, Harada S, Matsumoto C, Takita M, Miyaura C, Inada M
  • Journal Title: J.Health Sci. 55(5) Pages: 832-837
  • Peer Reviewed
• [Journal Article] Hyaluronan inhibits bone resorption by suppressing prostaglandin E synthesis in osteoblasts treated with interleukin-1. (2009)
  • Author(s): Hirata M, Kobayashi M, Takita M, Matsumoto C, Miyaura C, Inada M
  • Journal Title: Biochem.Biophys.Res.Commun. 381 Pages: 139-143
  • Peer Reviewed
• [Journal Article] Naringin suppresses osteoclast formation and enhances bone mass in mice. (2009)
  • Author(s): Hirata M, Matsumoto C, Takita M, Miyaura C, Inada M
  • Journal Title: J.Health Sci. 55(3) Pages: 1-5
  • Peer Reviewed
• [Journal Article] A novel carborane analog, BE360, with a carbon-containing polyhedral boron-cluster is a new selective estrogen receptor modulator for bone. (2009)
  • Author(s): Hirata M, Inada M, Matsumoto C, Takita M, Ogawa T, Endo Y, Miyaura C
  • Journal Title: Biochem.Biophys.Res.Commun. 380 Pages: 218-222
  • Peer Reviewed
• [Journal Article] 1α,25-Dihydroxyvitamin D_3-26,23-lactam, a novel vitamin D_3 analog, acts as a vitamin D_3 antagonist in human prostate cancer cells. (2008)
  • Author(s): Takita M, Hirata M, Tsukamoto K, Nagasawa K, Miyaura C, Inada M
  • Journal Title: J.Health Sci. 54(4) Pages: 497-502
  • Peer Reviewed
• [Journal Article] Novel vitamin D_3 analogs, 1α,25(OH)_2D_3-26,23-lactam (DLAMs), antagonize bone resorption via suppressing RANKL expression in osteoblasts. (2008)
  • Author(s): Inada M, Tsukamoto K, Hirata M, Takita M, Nagasawa K, Miyaura C
  • Journal Title: Biochem.Biophys.Res.Commun. 372 Pages: 434-439
  • Peer Reviewed
• [Journal Article] Matrix metalloproteinases and bone. (2008)
  • Author(s): Krane SM, Inada M
  • Journal Title: Bone 43 Pages: 7-18
  • Peer Reviewed
• [Presentation] Role of prostaglandin E receptor EP4 in breast cancer growth and osteolysis due to bone metastasis, tumor growth and bone metastasis of cancer (2010)
  • Author(s): Yokoyama S, Takita M, Maruyama T, Miyaura C, Inada M
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: Toronto, Ontario, Canada
  • Year and Date: 2010-10-17
• [Presentation] Disruption of prostaglandin E biosynthesis and Its receptor EP4 attenuated angiogenesis, tumor growth and bone metastasis of cancer (2010)
  • Author(s): Takita M, Yokoyama S, Inada M, Miyaura C
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: Toronto, Ontario, Canada
  • Year and Date: 2010-10-16
• [Presentation] Prostaglandin E2 promotes bone metastasis and osteolysis via PGE receptor EP4 but not EP1 and EP3 in host cells. (2009)
  • Author(s): Takita M, Inada M, Yokoyama S, Maruyama T, Miyaura C
  • Organizer: The American Society of Bone and Mineral Research
  • Place of Presentation: Colorado, USA
  • Year and Date: 2009-09-14
• [Presentation] Hyaluronan attenuated IL-1 induced prostaglandin E2 production and osteoclast. differentiation via CD44 mediated NFκB inactivation in osteoblast. (2009)
  • Author(s): Hirata M, Kobayashi M, Tominari T, Matsumoto C, Miyaura C, Inada M
  • Organizer: American Society of Bone and Mineral Research
  • Place of Presentation: Colorado, USA
  • Year and Date: 2009-09-13
• [Presentation] Carborane BE360, one of the carbon-containing polyhedral boron-cluster compounds, is a new type of selective estrogen receptor modulator. (2008)
  • Author(s): Inada M, Tominari T, Hirata M, Matsumoto C, Takita M, Endo Y, Miyaura C
  • Organizer: American Society of Bone and Mineral Research
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2008-09-12
• [Presentation] (Ala-Gly)n sequences configured silk protein scaffold induces osteoblast differentiation and bone formation via increased cbfa1 and osterix expression. (2008)
  • Author(s): Hirata M, Kobayashi M, Yoshida A, Miyaura C, Asakura T, Inada M
  • Organizer: American Society of Bone and Mineral Research
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2008-09-12
• [Book] Clinical Calcium 21(卵巣摘出動物および精巣摘出動物) (2011)
  • Author(s): 稲田全規、宮浦千里
  • Total Pages: 164-170
• [Book] Clinical Calcium 20(サイトカインと閉経後骨粗鬆症) (2010)
  • Author(s): 稲田全規、宮浦千里
  • Total Pages: 19-24
• [Book] 細胞41(性ホルモンの骨作用と骨疾患) (2009)
  • Author(s): 稲田全規、松本千穂、宮浦千里
  • Total Pages: 12-15
• [Patent(Industrial Property Rights)] 歯周病発症の評価システム (2011)
  • Inventor(s): 宮浦千里、稲田全規
  • Industrial Property Rights Holder: 国立大学法人東京農工大学
  • Industrial Property Number: 特許,特許第4662043号
  • Filing Date: 2011-01-14