2010 Fiscal Year Final Research Report
Analysis of a novel protein complex involved in RNAi and maintenance of chromosome integrity in higher eukaryote
| Project/Area Number |
20590056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
EKI Toshihiko Toyohashi University of Technology, 大学院・工学研究科, 教授 (40192512)
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| Project Period (FY) |
2008 – 2010
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| Keywords | RNA干渉 / 線虫 / 染色体 / ヘリカーゼ |
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| Research Abstract |
This study has aimed to clarify the molecular function of a novel nematode RNAi factor DRH-3 that was originally found in my previous work. In this study, I successfully identified a novel E1 protein that interacts with DRH-3 by comprehensive yeast two-hybrid analyses, suggesting that DRH-3 likely functions in the RNAi pathway by interacting with E1 protein in C. elegans. Thus, E1 protein could be an important clue to clarify the molecular mechanism of the DRH-3-containing protein complex. In addition, the expression profile analyses suggested that DRH-3 localizes in particular cellular domains and plays a role in germ-line formation.
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