2010 Fiscal Year Final Research Report
Development and clinical application of antibody epitope peptide vaccines for personalized tumor immunotherapy.
| Project/Area Number |
20590063
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
ITOH Kunihiko University of Shizuoka, 薬学部, 教授 (90221770)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Hideki 静岡県立大学, 薬学部, 講師 (00419665)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MASAKI Yasufumi 金沢医科大学, 医学部, 准教授 (40238895)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | オーダーメイド医療 / 癌治療 / エピトープワクチン / 抗体医薬 / ファージディスプレイ |
|---|
| Research Abstract |
I performed the epitope analysis of human recombinant Fab (AHSA) retrieved from a patient with MALT lymphoma using phage display random peptide library. AHSA-derived epitope peptide vaccines were given to BALB/c mice and then antibody Fab libraries were constructed from immunized spleen cells. Selected Fab clones show the same reactivity as AHSA against target antigen. Moreover, they showed the binding affinity stronger than that of AHSA. These results suggest that peptide vaccines from the epitope or mimotope of antibody medicines may serve as a effective inducers of highly specific and reactive anti-tumor antibody repertoires. Clinical applications of epitope peptide vaccines are now in progress.
|
-
-
[Journal Article] Generation of AcGFP-labeled single chain Fv against 5-methyl-2'-deoxycytidine from a hyperimmunized mouse using phage display technology.2010
Author(s)
Ohshima, M., Inoue, K., Hayashi, H., Tsuji, D., Mizugaki, M., Itoh, K.
-
Journal Title
Prot.Eng.Des.Select. 23
Pages: 881-888
Peer Reviewed
-
-
[Journal Article] Antibody epitope peptides as potential inducers of IgG antibodies against CD98 oncoprotein.2009
Author(s)
Itoh, K., Ohshima, M., Sonobe, M., Saito, M., Yoshida, A., Hayashi, H., Inoue, K., Masuko, T.
-
Journal Title
Cancer Sci 100
Pages: 126-131
Peer Reviewed
-
-
-
-
-
-
-
