2010 Fiscal Year Final Research Report
Role of poly(ADP-ribosyl) ation in spermatogonial stem cells
| Project/Area Number |
20590076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Otani Womens University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Seigo 大阪大谷大学, 薬学部, 教授 (70263150)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 精子幹細胞 / ポリ(ADP-リボシル)化 / PARP / 発生・分化 / 再生医療 |
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| Research Abstract |
Spermatogonial stem cells(SSCs) can proliferate in vitro in the presence of glial cell line-derived neurotrophic factor, a self-renewal factor for SSCs. These cultured SSCs are called germline stem(GS) cells. Growth rate, morphology, karyotype, DNA methylation profile and fertility of GS cells appear to be unaltered during a 2-year culture, revealing remarkable stability of GS cells. In this study, we investigated the role of poly(ADP-ribosyl) ation in GS cells. Poly(ADP-ribosyl) ation is a posttranslational modification of proteins mediated by poly(ADP-ribose) polymerase(PARP) which is involved in genomic stability. We examined the effect of PARP inhibitor on GS cells. PARP inhibition reduced growth rate of GS cells, and induced cell death. Analyses of gene expression patterns showed that PARP inhibition reduced DNA methyltransferase genes expression. These results suggest that poly(ADP-ribosyl) ation contribute to maintenance of DNA methylation in GS cells.
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[Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects2009
Author(s)
Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T
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Journal Title
Biol Reprod
Volume: 81
Pages: 155-64
Peer Reviewed
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