2010 Fiscal Year Final Research Report
Intermolecular network involved in calcium-dependent apoptosis of pancreatic beta-cells and possible targets for diabetes treatment
| Project/Area Number |
20590085
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
ISHIKAWA Tomohisa University of Shizuoka, 薬学部, 教授 (10201914)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANEKO Yukiko 静岡県立大学, 薬学部, 助教 (00381038)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 膵β細胞 / アポトーシス / カルシウム / ミトコンドリア / 酸化ストレス |
|---|
| Research Abstract |
We investigated mechanisms for apoptosis induction in pancreatic beta-cells, which can be responsible for impairment of insulin secretion in type 2 diabetes. The treatment of beta-cell line INS-1 with H_2O_2, which resembled diabetic oxidative stress, induced a transient intracellular Ca^<2+> elevation. The response was mediated by Ca^<2+> release from the endoplasmic reticulum through IP3 receptors and terminated within one hour. This seemed to become a trigger signal for beta-cell apoptosis induction ; the transient cytosolic Ca^<2+> elevation led to prolonged elevation of mitochondrial Ca^<2+> concentration, thereby inducing cytochrome c release from mitochondria and beta-cell apoptosis.
|
