2010 Fiscal Year Final Research Report
Construction of artificial poly-clonal gamma globulin to control HCV infection and development of assay system
| Project/Area Number |
20590117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | National Institute of Biomedical Innovation |
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Principal Investigator |
KAMEOKA Yousuke National Institute of Biomedical Innovation, 難病・疾患資源研究部・難病資源研究室 (00224692)
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| Co-Investigator(Kenkyū-buntansha) |
OOSHIMA Masamiti 国立感染症研究所, 免疫部, 室長 (10272428)
SUZUKI Kazuo 千葉大学, 医学研究科, 特任教授 (20192130)
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| Project Period (FY) |
2008 – 2011
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| Keywords | ゲノム創薬 / 人体抗体 / C型肝炎 / HCV / 感染制御 |
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| Research Abstract |
178 clones of artificial poly-clonal gamma globulin, which consist of VH, CH1 and hinge region, was constructed from cDNA originated from peripheral blood. The expressed recombinant proteins in E. coli, were extracted, purified and recovered in milligram order. SCGK/J mouse, which is an inflammatory disease model, although the strain is not a model of hepatic disease, were examined for therapeutic treatment with the recombinant poly-clonal gamma globulin. After the therapeutic treatment in some dose of recombinant protein, hemogram and some cytokines were assayed. The level of peripheral blood lymphocyte and some inflammatory cytokines were decreased in dose dependent manner, and some suppressive cytokines were increased. It was suggested that the recombinant poly-clonal gamma globulin, constructed in this study, would be possible to apply to the therapy for hepatic inflammatory disease.
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Research Products
(19 results)
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[Journal Article] Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus.2009
Author(s)
Osada N, Hirata M, Tanuma R, Suzuki Y, Sugano S, Terao K, Kusuda J, Kameoka Y, Hashimoto K, Takahashi I.
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Journal Title
BMC Res Notes. 2
Pages: 199
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[Journal Article] Large-scale analysis of Macaca fascicularis transcripts and inference of genetic divergence between M.fascicularis and M.mulatta.2008
Author(s)
Osada N, Hashimoto K, Kameoka Y, Hirata M, Tanuma R, Uno Y, Inoue I, Hida M, Suzuki Y, Sugano S, Terao K, Kusuda J, Takahashi I.
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Journal Title
BMC Genomics 92
Pages: 90-
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[Journal Article] Preparedness for the spread of influenza: prohibition of traffic, school closure, and vaccination of children in the commuter towns of Tokyo.2008
Author(s)
Yasuda H, Yoshizawa N, Kimura M, Shigematsu M, Matsumoto M, Kawachi S, Oshima M, Yamamoto K, Suzuki K.
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Journal Title
J Urban Health. 85
Pages: 619-635
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[Journal Article] A comparative study of the diagnostic accuracy of ELISA systems for the detection of anti-neutrophil cytoplasm antibodiesavailable in Japan and Europe.2008
Author(s)
T.Ito-Ihara, E.Muso, S.Kobayashi, K.Uno, N.Tamura, Y.Yamanishi, A.Fukatsu, R.A.Watts, D.G.I.Scott, D.R.W.Jayne, K.Suzuki, H.Hashimoto.
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Journal Title
Clin.Exp.Rheumatol. 26(6)
Pages: 1027-33
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